The potential role of P.gingivalis in gastrointestinal cancer: a mini review
Infectious Agents and Cancer volume 14, Article number: 23 (2019)
Bacterial infection may be involved in the entire process of tissue carcinogenesis by directly or indirectly affecting the occurrence and development of tumors. Porphyromonas gingivalis (P.gingivalis) is an important pathogen causing periodontitis. Periodontitis may promote the occurrence of various tumors. Gastrointestinal tumors are common malignant tumors with high morbidity, high mortality, and low early diagnosis rate. With the rapid development of molecularbiotechnology, the role of P.gingivalis in digestive tract tumors has been increasingly explored. This article reviews the correlation between P.gingivalis and gastrointestinal cancer and the pathogenesis of the latter. The relationship among P.gingivalis, periodontal disease, and digestive tract tumors must be clarifiedthrough a multi-center, prospective, large-scale study.
Pathogen infection is one of the main causes of chronic inflammation, and the most common types are bacterial and viral infections . With the development of clinical medicine, a fundamental has shift occurred in the link between bacteria and cancer . Tumorigenesis may be associated with inflammation. Bacterial infection may participate in the entire process of carcinogenesis by directly or indirectly affecting the occurrence and development of the disease.  Chronic infection may be involved in 17~28% of the morbidity and death of patients with cancer in countries such as China and South Korea .
The human flora plays an important role in normal physiological activities , and carcinogenesis . More than 700 kinds of bacteria exist in the normal oral cavity, and these include at least 11 mycobacteria and 70 genera , among which red complex is an important pathogenic bacterium for periodontal disease . Red complex consists of P.gingivalis, Treponema denticola (T. denticola) and Tannerellaforsythia . All three are Gram-negative anaerobic bacteria, which can express virulence factors to interfere in the defense system,and invade and destroy periodontal tissue and host immune function .
The morbidity rate of periodontitis is approximately 10.0~17.6%, and it is often accompanied with increasing levels of inflammatory factors . The definition “chronic periodontitis” has been replaced in the new periodontal diseases classification , the forms of the disease previously recognized as “chronic” or “aggressive”, now grouped under a single category, “periodontitis”. In this study we have made changes according to the latest standards. Pathogenic bacteria can spread to the blood and even to the brain , and they are associated with various of systemic diseases and cancers [11, 12]. Periodontitis can cause tooth loss, and meta-analyses  suggest that tooth loss and alimentary canal cancer are remarkbaly correlated and exhibit a dose–effect relationship, that is, the overall risk of cancer increases by 9% for every 10 teeth lost, and esophageal cancer (+ 14%), gastric cancer (+ 9%), head and neck cancer (+ 31%), colorectal cancer (+ 4%) and pancreatic cancer (+ 7%) .
P.gingivalis is an important pathogenic bacterium that mediates the local inflammatory response of periodontitis , adhere to and invade gingival epithelial cells, interfere with normal physiological metabolism, and inhibit apoptosis , which is a potential risk factor for cancer . Gastrointestinal cancer involves common malignant tumors with high morbidity and mortality, low diagnostic rate in the early stage, huge consumption of medical resources , high treatment cost for patients at the late stage, and poor effects. Furthermore, the early diagnosis rate of digestive tract tumors is low [18, 19], and advanced treatment costs are high and have poor effects, therefore tumors have become the main healthcare burden of Chinese residents . Domestic and foreign researches have shown a correlation between P. gingivalis and digestive tract tumor [21, 22]. This article systematically evaluates the results of recent studies to provide clinical assistance.
With regard to P. gingivalis
P. gingivalis is a gram-negative obligate anaerobic bacillus , which can express a variety of virulence factors, including trichoderma, gingipains, tetratricopeptide repeat (TPR) sequence protein, extracellular polysaccharides, hemoglobin uptake system, lipopolysaccharides (LPS), etc. . Gingipains and LPS co-activate could affect periodontal tissue immune defense function, and cause inflammation, leading to periodontal tissue destruction and alveolar bone absorption .
Gingipains can degrade foreign protein and polypeptide, which provide nutrition to P. gingivalis and maintain its growth . P. gingivaliscan produce virulence factors, such as Outer membrane vesicles (OMVs), E-cadherin, toxin and proteolytic enzyme . P. gingivalis OMVs show enriched selectivity in C-terminal domain (CTD)-family proteins, support bacterial cohesion, promote the development of the biological membrane, and function as intermdiates for transporting nonmotile bacteria .
As an intracellular pathogenic bacterium, P. gingivalis can invade a variety of eukaryotic cells,such as human aortic endothelial cells, human coronary artery endothelial cells (HCAEC) , human umbilical vein endothelial cells , gingival epithelial cells , coronary artery smooth muscle cells (CASMC) , epithelial buccal KB cells . After invasion, P. gingivalis changes its expressionto avoid the immune defenses of the host and then serves as a reservoir for future reinfection [26, 28]. In addition, it can interact with the host and colonize periodontal tissue .
On the basis of different antibody levels of P. gingivalis IgG in vivo, periodontitis is divided into none or light(<69EU(Enzyme-linked immunosorbent assay unit)),medium (69.1–119.0 EU) and severe (> 119.0 EU) [31, 32]. After adjusting for risk factors such as age and gender, etc., Ahn J et al  discovered that patients with periodontitis exhibit increased cancer mortality (RR = 2.42,95% CI =1.48–3.95). Moreover,the risk still increases even though risk factors, such as smoking, education, race/ethnicity and body mass index are further controlled (RR = 2.28,95% CI = 1.17–4.45), the risk increases with the severity of periodontitis. The mortality rate of patients with periodontitis generally increases with the increase in P.gingivalis IgG level . However, the study in Taiwan  found that after adjusting the known risk factors, patients with severe periodontitis do not show an increased overall risk of gastrointestinal tumor (HR: 0.99, 95% CI: 0.84–1.16) nor the risk of a single tumor such as esophageal cancer, gastric cancer, small intestinal tumor, colorectal cancer and pancreatic cancer. The reasons for the different findings may be related to differences in disease severity, race and sample size, and differences in risk factors between the two studies.
P. gingivalis and oral squamous cell carcinoma (OSCC)
Oral cancer is the sixth most common cancer in the world and one of the most common cancers in developing countries . OSCC is the most common type of oral cancer with high incidence and recurrence rates and poor prognosis [36, 37]. Patients often experience logopathy and swallowing dysfunction, which seriously affect facial appearance and social interactions . The specific risk factors of OSCC include dietary habits, poor living habits, such as smoking, alcohol abuse, betel nut chewing, DNA carcinogenic virus and genetic factors [39,40,41],; however, OSCC in approximately 15% of patients still cannot be explained by these factors .
Studies have shown that oral flora plays a role in oral cancer . Oral bacterial infection can cause chronic periodontal inflammation, and serum P.gingivalis IgG levels are significantly increased in patients with periodontitis compared with normal subjects [31,32,33]. Pathogenic bacteria such as P. gingivalis have been detected in OSCC , indicating that periodontitis is probably related to OSCC. After long-term and repeated exposure to P. gingivalis, the invasiveness of OSCC cells increases either by IL-8 and MMPs upregulation of  or by obtaining stem cell characteristics .
After continuous exposure to P. gingivalis, human immortalized oral epithelial cells show improved proliferation invasiveness and tumorigenicity improve . The possibility of carcinogenesis increases after the primary cell of a human being is infected by P. gingivalis . In addition, continuous infection with P. gingivalis causes paclitaxel resistance in OSCC cells, and improved metastatic ability . The relationship of OSCC with P. gingivalis, as a periodontitis -dominant bacterium, is a research hotspot that has been extensively reviewed by many researchers [42, 50,51,52]. Thus, it is a topic that will not be repeated in this paper.
P. gingivalis and esophageal cancer (EC)
EC includes two main types: esophageal adenocarcinoma (ECA) and esophageal squamous cell carcinoma (ESCC), which is dominated by ECA in Western countries, while ESCC is predominant in Eastern countries . EC is the third most common cancer in China, with the fourth highest mortality rate . EC may be related to oral microecology, and Chinese population-based study  has shown that poor oral hygiene is closely related to the development of ESCC. A retrospective study in Taiwan  revealed that the risk of obtaining EC decreases after the prevention of periodontitis in men (HR = 0.54,95% CI = 0.44–0.66), but no significant statistical difference has been observed in women. Peters B A et al  further revealed the increasing P. gingivalis can increase the risk of getting ESCC.
Chinese scholars Gao et al  discovered that the positive rates of P. gingivalis in ESCC, adjacent tissues and normal esophageal tissues were 61, 12% and 0, respectively. P. gingivalis infection is predicted to be the dominant factor in esophageal epithelial carcinogenesis and is positively correlated with clinical pathological features such as ESCC tissue differentiation status, metastasis and overall survival . The content of P. gingivalis IgG and IgAin 96 ESCC, 50 (esophagitis, EI) and 80 healthy controls serum samples were measured . The sensitivity/specificity of P. gingivalis IgG, IgA, and IgG+ IgA for the diagnosis of ESCC is 29.17%/96.90, 52.10%/70.81, and 68.75%/68.46%, respectively. P. gingivalis IgA exhibits better diagnostic performance for early ESCC than IgG (54.54% vs. 20.45%). In addition, ESCC patients with high levels of P. gingivalis IgG or IgA have poor prognosis, especially those with stage 0-II or lymph node metastasis; furthermore, patients have positive P. gingivalis IgG+ IgA present poor prognosis . Thus, P. gingivalis may be used for ESCC screening and prognosis monitoring, and is conducive to the early detection of ESCC [58, 59].
P. gingivalis and gastric cancer (GC)
GC is the fourth most prevalent malignant tumor worldwide, and its pathogenesis is a multi-factor, multi-stage process . Studies on the relationship between GC and precancerous lesions have produced inconsistent findings. A meta-analysis  showed that tooth loss may be a risk factor for GC. Periodontal pathogen colonization is associated with increased risk of precancerous lesions of gastric cancer , thereby suggesting that periodontal pathogens may be associated with GC.
Chronic atrophic gastritis (CAG), intestinal metaplasia (IM) and dysplasia belong to PLGC [63, 64]. In the cross-sectional study by Salazar C R et al  on 37 patients with dysplasia, IM or dysplasia and 82 controls. The results showed that the colonization of P. gingivalis, A. actinomycetemcomitans and T. denticola in plaque increased, but it was inconsistent with the DNA level of PLGC. After adjusting for risk factors such as age and gender, Ahn JY et al  reported no increase in P. gingivalis IgG level in GC (RR = 1.02,95% CI: 0.40–2.55), and this result is similar to the findings when other factors, such as smoking and education, are controlled (RR = 0.99; 95% CI: 0.38–2.58). Yuan et al  discovered that P. gingivalis cannot survive in environments with high acidity and decreases with increasing acid levels and bacterial flora formation, thereby causing low infection of P. gingivalis in the stomach and cardia. The relationship between P. gingivalis and GC requires further study.
P. gingivalis and hepatocellular carcinoma (HCC)
HCC is one of the most common malignancies  and is the third leading cause of cancer death worldwide . In the vast majority of HCC, viruses and toxic metabolites are observed to cause chronic damage and inflammation, leading to liver fibrosis/cirrhosis . Up till now,risk factors for about 25% HCC are still unknown ,and bacterial infections may play a role in the development of HCC .
Japanese researchers  discovered thatpatients with HCC and merged periodontitis have higher Japan integrated stage (JIS) scores and reactive oxygen species (ROS) levels than HCC patients without periodontitis. Han et al  reported that periodontitis may participate in the progression of liver diseases, such as nonalcoholic fatty liver disease,cirrhosis, HCC and liver transplantation, but the correlation between periodontitis and liver diseases remains unclear. A prospective cohort study in Finland  found that tooth loss among male smokers was correlated with HCC morbidity risk. Compared with patients who have lost 0–10 teeth, those who have lost 11–31 permanent tooth have significantly increased HCC risk (HR 1.42, 95% CI 1.01–1.98). The risk was higher for patients who lost more teeth (HR 1.45, 95% CI 1.00–2.10).
P. gingivalis is a pathogenic bacterium that possibly participates in liver diseases formation. In 2009, Nishihara R et al  discovered that after KKAy diabetes mice are inoculated with P. gingivalis, their blood glucose, serum tumor necrosis factor alpha (TNF-α), and interleukin − 6 (IL-6) are significantly increased,whereas adiponectin is significantly reduced to 35.7%. These findings are similar to results on liver tissues . After treatment with anti-TNF-α antibody, the reaction of KKAy mice to P. gingivalis improves, and the lesion size after inoculation is reduced . Moreover, Takano M et al  stimulated mouse hepatoma cell Hepa-1.6 with P. gingivalis solution and increased the cell TNF-α and IL-6 level; the cell level is significantly decreased after treatment with anti-TNF-α antibody.
Nagao Y et al  discovered a positive fimbrillin (fimA) genotype of P. gingivalis in patients with hepatitis C (HC) and proposed that periodontitis may be related to the progression of HC; furthermore, controlling oral diseases may be helpful incontrolling hepatic fibrosis. In addition, gingivalislipid Aexhibits anti-MH134 liver cancer activity in C3H/HEN mice .P. gingivalis possibly participates in hepatic pathological changes through TNF-α and IL-6. However, whether HCC exerts a promoting or restraining effect requires further study.
P. gingivalis and colorectal cancer (CRC)
The relationship between periodontitis and CRC remains unclear. The study of Michaud DS et al  on medical staff showed that the tumor morbidity of male patients with periodontitis is increased(+ 13%), whereas patients with severe periodontitis (remaining teeth < 17) exhibit high morbidity rates(+ 45%). Non-smoking male patients with periodontitis have an increased risk of esophageal and head and neck cancer, whereas CRC displays no change . However, Ahn J et al  reported that periodontitis is related to the increased in risk of death in patients with CRC. After age and gender risk factors are adjusted, the risk of death from CRC increases (RR = 4.34; 95% CI:1.31–14.44) and remains high after adjusting for smoking, education, race /ethnicity and BMI. (RR = 3.58; 95% CI:1.15–11.16).
Purcell RV and his colleagues , for the first time, connected a single bacterium with tumour consensus molecular subtypes (CMS). Their results demonstrated that P. gingivalis and Trichoderma horseshoe are correlated with CMS1, and F.nucleatum, Parvimonasmicra and Peptostreptococcusstomatis are abundant in CMS1. Therefore, P. gingivalis is candidate bacterium of CRC, but further study is required to verify the correlation.
P. gingivalis and pancreatic cancer (PC)
PC risk factors include smoking, obesity and type 2 diabetes; however, these factors are insufficient to explain the 50% cause of morbidity . A study found that patients with PC often have unbalanced intestinal microbiota . Compared with healthy flora, changes occur in the oral cavity and intestinal and pancreatic tissues of patients with PC ,indicating a relationship between microecology and PC . Moreover, inflammation may play a role in the development of PC, but the specific mechanism is still unclear .
Changes in oral bacterial flora are probably related to the increase in PC morbidity. People with poor oral health have an increased risk of PC disease [81,82,83], and new research suggests that pathogens may play a role in PC . If this assumption is established, the real cause of pancreatic cancer may be unveiled . The authors [85, 86] speculate that peptide-based arginine deaminase (PAD) enzymes secreted by oral bacterial flora including P. gingivalis, Forsythia bacillus and Treponema pallidum probably cause the gene mutation of P53 and K-ras, which in turn trigger PC. After adjusting for age and gender risk, patients with periodontal diseases present an increased risk of death from PC (RR = 4.99; 95% CI:1.11–22.5), and the risk remains elevated after further adjustment for factors such as smoking, education, race/ethnicity, and BMI (RR = 4.56; 95% CI:0.93–22.29, 34].
Torreshas et al  detected the levels of salivary bacteria of patients with PC, identifying 12 eumycota and 139 genus, in which five eumycot as Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes and Fusobacteriaaccounted for 99.3% of the oral bacteria. The proportion of Leptotrichia and Porphyromonas in the saliva of patients with PC was higher than that in the saliva of healthy controls (P < 0.001).
Epidemiological data suggests that P. gingivalis may play a role in the pathogenesis of PC . The Fan et al  prospective nested case-control study showed that the abundance of. P. gingivalis and Actinobacillus is increased, whereas that of Fusobacterium and Leptotrichia is reduced due to the increased PC risk. Michaud et al  prospectively investigated the correlation between periodontal antibodies to pathogen and PC risks, and their results revealed that high levels (> 200 ng/ml) of P. gingivalis antibody are associated with a twofold increase in PC risk compared with low levels(≤200 ng/ml);furthermore the risk of PC is doubled (OR = 2.14,95% CI, 1.05–4.36), thereby suggesting that periodontitis may increase the risk of PC.
P. gingivalis and other gastrointestinal cancers (gallbladder cancer, cholangiocarcinoma, small intestinal tumors, etc.)
No studies related to periodontitis and cancer have been found in the previous literature. Chou et al  first reported that, the risk of cancer for patients with medium periodontitis does not increase compared with that for patients with slight periodontitis (HR = 0.74,95% CI: 0.23–2.34). However, his study was limited by the small sample size. The overall survival rate of Extrahepatic cholangiocarcinoma was greatly lower than those of other cancers .Meta-analysis  showed that the risk of gallbladder cancer was associated with Salmonella typhi, and no related reports on P. gingivalis and extrahepatic cholangiocarcinoma were found. Whether P. gingivalis is related to the above tumors requires further study.
Conclusion and future studies
Severe periodontitis, is an important risk factor of gastrointestinal cancer that severely threatens human health. Controlling periodontitis contributes to early cancer prevention. P. gingivalis is probably an important risk factor of gastrointestinal cancer, especially for oral cancer, esophageal cancer, colorectal cancer and pancreatic cancer (Fig. 1). Investigations on the relationship among P. gingivalis, periodontitis, and gastrointestinal cancer are crucial, but multicenter, prospective, and large-sample studies are necessary.
To date, many studies have supported that periodontitis is a potential risk factor of human diseases. The morbidity rates of periodontal infection and systemic disease have remarkably increasedin previous decades, and the correlation between the diseases and pathogenesis remains unclear . Recently, scientist  showed that microorganisms can be used for cancer treatment, but whether P. gingivalis is beneficial to human health remains unclear. The pathogenic mechanism of P. gingivalis must be further explored to provide a new direction for cancer prevention and treatment, and to improve the health level and quality of life of patients suffering from this disease.
Availability of data and materials
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We sincerely thank professor Guo-jian Xie from department of gastroenterology of Taihe hospital for her unselfish help in this study.
This study was funded by the doctor’s start-up fund of Taihe Hospital (2012QD09),and the 2016 Joint Diagnostic Medicine Research Project of Taihe Hospital (2016JZ02).
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Liu, Xb., Gao, Zy., Sun, Ct. et al. The potential role of P.gingivalis in gastrointestinal cancer: a mini review. Infect Agents Cancer 14, 23 (2019). https://doi.org/10.1186/s13027-019-0239-4