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High viral load of Merkel cell polyomavirus DNA sequences in Langerhans cell sarcoma tissues

  • Ichiro Murakami1Email author,
  • Michiko Matsushita2,
  • Takeshi Iwasaki1,
  • Satoshi Kuwamoto1,
  • Masako Kato1,
  • Yasushi Horie3,
  • Kazuhiko Hayashi1,
  • Jean Gogusev4,
  • Francis Jaubert5,
  • Shu Nakamoto6,
  • Mitsunori Yamakawa7,
  • Hirokazu Nakamine8,
  • Katsuyoshi Takata9,
  • Takashi Oka9 and
  • Tadashi Yoshino9
Infectious Agents and Cancer20149:15

https://doi.org/10.1186/1750-9378-9-15

Received: 7 January 2014

Accepted: 28 March 2014

Published: 6 May 2014

Abstract

Background

Langerhans cell (LC) sarcoma (LCS) is a high-grade neoplasm with overtly malignant cytologic features and an LC phenotype. We very recently suggested that LC behaves as a reservoir for common dermotropic Merkel cell polyomavirus (MCPyV) and determined the relationship between LC histiocytosis (LCH), which has an underlining oncogenic capacity, and MCPyV as a trigger for a reactive process rather than a neoplastic process. We propose LC to be a reservoir for MCPyV and hypothesize that some LCS subtypes may be related to the MCPyV agent.

Findings

We examined seven LCS tissues using multiplex quantitative PCR (Q-PCR) and immunohistochemistry with anti MCPyV large-T (LT) antigen antibody. High viral loads of MCPyV DNA sequences (viral load = relative levels of MCPyV) were detected (0.328–0.772 copies/cell (Merkel cell carcinoma (MCC) = 1.0)) using Q-PCR in 43% (3/7) tissues, but LT antigen expression was not observed (0/7).

Conclusions

Frequent MCPyV-DNA amplification suggests that LCS in some patients may be related to MCPyV infection. Moreover, the higher viral load of LCS (median, 0.453 copies/cell) than low load of LCH (0.003, median of 12 cases) (P < 0.01) may suggest a virally induced tumorigenic process in some LCS. Although the absence of LT antigen expression may indicate a different role for MCPyV in this pathology, some subtypes of LCS may develop in the background of MCPyV-infected LC. To the best of our knowledge, this is the first report on the relationship between MCPyV and LCS. The recent discovery of MCPyV opened new therapeutic avenues for MCC. These data open novel possibilities for therapeutic interventions against LCS.

Keywords

Merkel cell polyomavirusLangerhans cell sarcomaLangerhans cellMultiplex quantitative PCR

Findings

Background

Merkel cell polyomavirus (MCPyV) was discovered in 2008 and was linked to the pathogenesis of Merkel cell carcinoma (MCC), which is a rare and aggressive skin cancer occurring in the dermis of individuals aged 60 years or older [1, 2]. Approximately 80% MCC harbors MCPyV, indicating its prominent role in the development of the disease. Mechanistically, MCPyV-induced oncogenesis is considered to be induced by MCPyV large T (LT) antigen through molecular binding with the retinoblastoma protein [1]. Several tumorigenic processes leading to MCC were proposed. One was that the induced mutations of MCPyV due to long exposure to ultraviolet light leads to integration of the cytoplasmic viral sequences into the DNA of originating MCC cells. MCPyV primarily resides on the skin, and we have detected MCPyV-DNA in the organs of autopsy cases, with the highest prevalence (53%) in the skin [3].

Because Langerhans cells (LCs) exist above the middle of the spinous zone of epidermis [4] and can capture external pathogens [5] and because of their ability to play roles as antigen-presenting cells [6, 7], we proposed that external pathogens may be initially recognized by LC and may subsequently infect Merkel cells which are mostly located at the basal cell layer of the epidermis. Therefore, we hypothesized that LC were a reservoir for MCPyV and demonstrated this phenomenon by showing MCPyV-DNA in microdissected LC of dermatopathic lymphadenopathy (DLA) [8].

In this study, we hypothesized that LC sarcoma (LCS) may originate from a long standing reservoir cell for MCPyV and showed some prevalence of MCPyV-DNA in LCS with high viral load compared with that in non-affected LC.

Results

Quantitative PCR (Q-PCR) for MCPyV-DNA in LCS tissues

The results of Q-PCR tissue analysis for MCPyV are shown in Table 1 with a positive (MCC = 1.0) and negative control (water = −). MCPyV-DNA sequences in three of seven tissues from LCS patients were detected corresponding to high viral loads (0.328–0.772).
Table 1

Clinical characteristics and lesional MCPyV data of patients with LCS

Patient

Age (years)

Sex

Tissue

Q-PCR (Viral load)*

MCPyV large T antigen (CM2B4)

LCS1

81

F

Skin

-

-

LCS2

73

M

LN

-

-

LCS3

72

F

Salivary gland

+ (0.453)

-

LCS4

61

M

Lung

+ (0.772)

-

LCS5

62

M

LN

+ (0.328)

-

LCS6

40

M

LN

-

-

LCS7

81

F

LN

-

-

The median age of the LCS patients (n = 7) was 72 years (range, 42–81 years of age). *Positive control (MCC):+ (Viral load = 1.0), Negative control (double distilled water): −. Viral load, relative levels of MCPyV. Abbreviations: LCS Langerhans cell sarcoma, LN lymph node, MCC Merkel cell carcinoma, MCPyV Merkel cell polyomavirus, Q-PCR multiplex quantitative PCR for MCPyV.

Immunohistochemistry for MCPyV-LT antigen

Neither cytoplasmic nor nuclear immuno-reactivity for this protein was observed in LCS cells as reported in Figure 1 and Table 1. MCC as a positive control is shown in Figure 1.
Figure 1

No immunoreactivity for MCPyV-LT using CM2B4 antibody in LCS tissue lesion (LCS4). Insert: MCC tissue lesion as a positive control. Scale bar, 50 μm. Abbreviations: CM2B4, a monoclonal antibody to MCPyV-LT; LCS, Langerhans cell sarcoma; MCC, Merkel cell carcinoma; MCPyV, Merkel cell polyomavirus; MCPyV-LT, large T antigen protein of MCPyV.

Discussion

LC is an epidermal dendritic cell (DC) with antigen-presenting cell capacities. Immature DC typically responds to pathogen exposure by specific maturation processes that facilitate induction of further innate and adaptive immune responses [6, 9]. However, some viruses, such as HIV, vaccinia, measles, and dengue, interfere with DC function and maturation to escape the immune surveillance [913].

Buffy coats of healthy donors above 20 years of age examined for MCPyV-DNA showed a 22% prevalence [14]. In this study [14] the cellular reservoir was not shown. In another investigation [15], CD14+ activated monocytes in peripheral blood were shown to serve as a reservoir for MCPyV. Recently we have described the prevalence of MCPyV in human tissues from 41 autopsy cases, i.e., skin (53%), lymph node (0%, excluding DLA), and lung (8%), with low viral load (viral load = 0.00026–0.22) [3]. Our data indicated the possibility that MCPyV is a dermotropic virus. We have previously shown the prevalence of MCPyV in LC in DLA tissues (viral load = 0.001–0.006) and in LC histiocytosis (LCH) lesions (viral load = 0.0001–0.033) [8]. Our data using DLA tissue, in which epidermal LCs with MCPyV migrate into lymph nodes [16, 17], suggest that LC is a candidate reservoir of MCPyV in the epidermis. MCPyV may also interfere with LC functions similar to some viruses, as mentioned above.

In this study, we have shown the presence of MCPyV in LCS with high viral loads (Table 1, viral load = 0.328–0.772). MCPyV-positive LCS samples were compared with MCPyV-positive LCH samples [8] in the point of MCPyV viral load in Figure 2 with significant difference (P < 0.01). The presence of MCPyV in LCS lesions suggests three possibilities: a) viral infection as a consequence of LCS development, b) LCS as a bystander, and c) a viral causal agent of LCS. Though MCPyV is typically an asymptomatic infection in adults [18], the presence of MCPyV with high viral loads denies the possibility it is a simple bystander within LCS lesions.
Figure 2

Q-PCR data comparing MCPyV-positive LCH samples and MCPyV-positive LCS samples. Q-PCR provides the viral load of MCPyV (relative level of MCPyV (MCC = 1)) for MCPyV-positive LCH samples and MCPyV-positive LCS samples plotted as box-whisker plots (Mann–Whitney U test, P < 0.01). The median viral load data are 0.003 and 0.453 in MCPyV-positive LCH and MCPyV-positive LCS, respectively. Abbreviations: LCH, Langerhans cell histiocytosis; LCS, Langerhans cell sarcoma; MCC, Merkel cell carcinoma; MCPyV, Merkel cell polyomavirus; Q-PCR, multiplex quantitative PCR.

Whether MCPyV-DNA is integrated into the nuclei of LCS cells as an oncogenic virus warrants further investigations, but the high viral loads of MCPyV in LCS lesions may suggest an important oncogenic factor in LCS cells. We are now trying specific PCR as Sastre-Garau et al. did [19]. Immunohistochemical negativity for MCPyV-LT (CM2B4) may indicate a different tumorigenetic mechanism that is involved in MCPyV-LT positive MCC [1, 20] or under the limit of detection for MCPyV-LT.

Conclusion

To the best of our knowledge, this is the first report indicating a relationship between MCPyV infection and LCS. Thus, we suggest that MCPyV may play some role as an oncogenic factor in particular subtypes of LCS. Based on the foregoing, we propose an LCS tumorigenesis model that MCPyV may be a cause of LCS. The recent discovery of MCPyV opened new therapeutic avenues for MCC [21]. Although MCPyV-LT expression was not detected, the origin of some forms of LCS may be MCPyV-infected LC. When confirmed, these findings will open novel possibilities for therapeutic interventions against LCS.

Methods

Patients and tissue samples of LCS

This study was approved by the Institutional Review Board of Faculty of Medicine, Tottori University, Yonago, Japan.

A total of seven tissues from patients with LCS were analyzed. All tissues of LCS were obtained as formalin-fixed paraffin-embedded (FFPE) samples.

Confirmation of accurate diagnosis of LCS

Diagnostic accuracy of all collected samples, histological sections and immunohistochemistry for CD1a, S100 protein (S100), and CD207 (langerin) of all specimens was confirmed by two pathologists on the basis of the diagnostic criteria [22].

DNA extraction from LCS tissues

DNA was extracted from each FFPE sample using the QIAamp DNA FFPE Tissue Kit and Mini Kit (QIAGEN GmbH, Hilden, Germany).

Multiplex quantitative PCR (Q-PCR) for MCPyV detection

Q-PCR was performed in a 10-μl reaction mix containing TaqMan® Copy number reference assay RNase P (Applied Biosystems, Foster City, CA, USA) as internal control [8]. A primer pair targeting the position 859–934 (MCPyV-LT) on MCC350 (GenBank EU375803) was [20] used. To determine the MCPyV-DNA ratio relative to MCPyV-DNA of the reference MCC (MCC = 1.0) for each case, Q-PCR was performed using an ABI PRISM 7900HT Sequence Detection System (Applied Biosystems) as previously described [20]. The ratio of the virus was determined using the viral signal in a positive MCC sample as a reference (viral load = relative levels of MCPyV, MCC = 1.0 copy/cell). Thresholds were plotted against each standard sample. All reactions of samples and controls were performed in triplicate, and the average was reported. The MCPyV-DNA ratio in each sample was determined on the basis of corresponding standard curves.

Immunohistochemistry for detection of MCPyV-LT antigen

For detection of MCPyV-LT expression, immunohistochemistry was performed using monoclonal antibody CM2B4 (mouse monoclonal IgG2b, 200 μg/ml, sc-136172, Santa Cruz Biotechnology, CA, USA) generated against a peptide fragment of MCPyV-LT as immunogen [23, 24].MCC samples were used as controls throughout.

Statistical analysis

Comparisons of MCPyV viral load between MCPyV-positive LCH and MCPyV-positive LCS were performed using the Mann–Whitney U test. Differences between values were considered statistically significant at P < 0.05.

Abbreviations

DC: 

Dendritic cell

DLA: 

Dermatopathic lymphadenopathy

FFPE: 

Formalin-fixed paraffin-embedded

LC: 

Langerhans cell

LCH: 

Langerhans cell histiocytosis

LCS: 

Langerhans cell sarcoma

LN: 

lymph node

MCC: 

Merkel cell carcinoma

MCPyV: 

Merkel cell polyomavirus

MCPyV-LT: 

Large T antigen protein of MCPyV

Q-PCR: 

Multiplex quantitative PCR.

Declarations

Acknowledgements

This work was partly supported by a Grant-in-aid for Scientific Research (C) 23590426 from the Japanese Ministry of Education, Science, Sports and Culture.

We thank Dr. Toshiharu Maeda (Ehime Prefectural Central Hospital) for providing clinical information and specimens.

Authors’ Affiliations

(1)
Division of Molecular Pathology, Faculty of Medicine, Tottori University, Yonago, Japan
(2)
Department of Pathobiological Science and Technology, School of Health Science, Faculty of Medicine, Tottori University, Yonago, Japan
(3)
Department of Pathology, Tottori University Hospital, Yonago, Japan
(4)
Inserm U507 and U1016, Institut Cochin, Paris, France
(5)
University of Paris Descartes (Paris V), Paris, France
(6)
Department of Pathology, Tottori Prefectural Central Hospital, Tottori, Japan
(7)
Department of Pathological Diagnostics, Yamagata University School of Medicine, Yamagata, Japan
(8)
Department of Laboratory Medicine, The Japan Baptist Medical Foundation, Kyoto, Japan
(9)
Department of Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

References

  1. Feng H, Shuda M, Chang Y, Moore PS: Clonal integration of a polyomavirus in human Merkel cell carcinoma. Science. 2008, 319: 1096-1100. 10.1126/science.1152586.PubMedPubMed CentralView ArticleGoogle Scholar
  2. Kuwamoto S: Recent advances in the biology of Merkel cell carcinoma. Hum Pathol. 2011, 42: 1063-1077. 10.1016/j.humpath.2011.01.020.PubMedView ArticleGoogle Scholar
  3. Matsushita M, Kuwamoto S, Iwasaki T, Higaki-Mori H, Yashima S, Kato M, Murakami I, Horie Y, Kitamura Y, Hayashi K: Detection of merkel cell polyomavirus in the human tissues from 41 Japanese autopsy cases using polymerase chain reaction. Intervirology. 2013, 56: 1-5. 10.1159/000338620.PubMedView ArticleGoogle Scholar
  4. Kanik A, Li M, Uramacher CD: Normal skin. Histology for pathologist. Edited by: Mills SE. 2012, Philadelphia: Lippincott Williams & Wilkins, 3-28.Google Scholar
  5. Kubo A, Nagao K, Yokouchi M, Sasaki H, Amagai M: External antigen uptake by Langerhans cells with reorganization of epidermal tight junction barriers. J Exp Med. 2009, 206: 2937-2946. 10.1084/jem.20091527.PubMedPubMed CentralView ArticleGoogle Scholar
  6. Banchereau J, Steinman RM: Dendritic cells and the control of immunity. Nature. 1998, 392: 245-252. 10.1038/32588.PubMedView ArticleGoogle Scholar
  7. Weitzman S, Egeler RM: Histiocytic disorders of children and adults: introduction to the problem, overview, historical perspective and epidemiology. Histiocytic disorders of children and adults. Edited by: Weitzman S, Egeler RM. 2005, Cambridge: Cambridge University Press, 1-13.View ArticleGoogle Scholar
  8. Murakami I, Matsushita M, Iwasaki T, Kuwamoto S, Kato M, Horie Y, Hayashi K, Imamura T, Morimoto A, Imashuku S, Gogusev J, Jaubert F, Takata K, Oka T, Yoshino T: Merkel cell polyomavirus DNA sequences in peripheral blood and tissues from patients with Langerhans cell histiocytosis. Hum Pathol. 2014, 45: 119-126. 10.1016/j.humpath.2013.05.028.PubMedView ArticleGoogle Scholar
  9. da Costa CET, Annels NE, Egeler RM: The immunological basis of Langerhans cell histiocytosis. Histiocytic disorders of children and adults. Edited by: Weitzman S, Egeler RM, Cambridge UK. 2005, Cambridge, UK: Cambridge University Press, 66-82.Google Scholar
  10. Grosjean I, Caux C, Bella C, Berger I, Wild F, Banchereau J, Kaiserlian D: Measles virus infects human dendritic cells and blocks their allostimulatory properties for CD4+ T cells. J Exp Med. 1997, 186: 801-812. 10.1084/jem.186.6.801.PubMedPubMed CentralView ArticleGoogle Scholar
  11. Engelmayer J, Larsson M, Subklewe M, Chahroudi A, Cox WI, Steinman RM, Bhardwaj N: Vaccinia virus inhibits the maturation of human dendritic cells: a novel mechanism of immune evasion. J Immunol. 1999, 163: 6762-6768.PubMedGoogle Scholar
  12. Tortorella D, Gewurz BE, Furman MH, Schust DJ, Ploegh HL: Viral subversion of the immune system. Annu Rev Immunol. 2000, 18: 861-926. 10.1146/annurev.immunol.18.1.861.PubMedView ArticleGoogle Scholar
  13. Izmailova E, Bertley FM, Huang Q, Makori N, Miller CJ, Young RA, Aldovini A: HIV-1 Tat reprograms immature dendritic cells to express chemoattractants for activated T cells and macrophages. Nat Med. 2003, 9: 191-197. 10.1038/nm822.PubMedView ArticleGoogle Scholar
  14. Pancaldi C, Corazzari V, Maniero S, Mazzoni E, Comar M, Martini F, Tognon M: Merkel cell polyomavirus DNA sequences in the buffy coats of healthy blood donors. Blood. 2011, 117: 7099-7101. 10.1182/blood-2010-09-310557.PubMedView ArticleGoogle Scholar
  15. Mertz KD, Junt T, Schmid M, Pfaltz M, Kempf W: Inflammatory monocytes are a reservoir for Merkel cell polyomavirus. J Invest Dermatol. 2010, 130: 1146-1151. 10.1038/jid.2009.392.PubMedView ArticleGoogle Scholar
  16. Shamoto M, Osada A, Shinzato M, Kaneko C, Yoshida A: Do epidermal Langerhans cells, migrating from skin lesions, induce the paracortical hyperplasia of dermatopathic lymphadenopathy?. Pathol Int. 1996, 46: 348-354. 10.1111/j.1440-1827.1996.tb03620.x.PubMedView ArticleGoogle Scholar
  17. O'Malley DP, George TI, Orazi A, Abbondanzo SL: Dermatopathic lymphadenitis. Atlas of nontumor pathology, First series, Fascicle 7, Benign and reactive conditions of lymph node and spleen. Edited by: King DW. 2009, Washington, DC: American registry of pathology, 143-145.Google Scholar
  18. Tolstov YL, Pastrana DV, Feng H, Becker JC, Jenkins FJ, Moschos S, Chang Y, Buck CB, Moore PS: Human Merkel cell polyomavirus infection II. MCV is a common human infection that can be detected by conformational capsid epitope immunoassays. Int J Cancer. 2009, 125: 1250-1256. 10.1002/ijc.24509.PubMedPubMed CentralView ArticleGoogle Scholar
  19. Sastre-Garau X, Peter M, Avril MF, Laude H, Couturier J, Rozenberg F, Almeida A, Boitier F, Carlotti A, Couturaud B, Dupin N: Merkel cell carcinoma of the skin: pathological and molecular evidence for a causative role of MCV in oncogenesis. J Pathol. 2009, 218: 48-56. 10.1002/path.2532.PubMedView ArticleGoogle Scholar
  20. Kuwamoto S, Higaki H, Kanai K, Iwasaki T, Sano H, Nagata K, Kato K, Kato M, Murakami I, Horie Y, Yamamoto O, Hayashi K: Association of Merkel cell polyomavirus infection with morphologic differences in Merkel cell carcinoma. Hum Pathol. 2011, 42: 632-640. 10.1016/j.humpath.2010.09.011.PubMedView ArticleGoogle Scholar
  21. Schrama D, Ugurel S, Becker JC: Merkel cell carcinoma: recent insights and new treatment options. Curr Opin Oncol. 2012, 24: 141-149. 10.1097/CCO.0b013e32834fc9fe.PubMedView ArticleGoogle Scholar
  22. Jaffe R, Weiss LM, Facchetti F: Tumours derived from Langerhans cells. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. Edited by: Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA, Stein H, Thiele J, Vardiman JW. 2008, Lyon, France: IARC, 358-360.Google Scholar
  23. Busam KJ, Jungbluth AA, Rekthman N, Coit D, Pulitzer M, Bini J, Arora R, Hanson NC, Tassello JA, Frosina D, Moore P, Chang Y: Merkel cell polyomavirus expression in merkel cell carcinomas and its absence in combined tumors and pulmonary neuroendocrine carcinomas. Am J Surg Pathol. 2009, 33: 1378-1385. 10.1097/PAS.0b013e3181aa30a5.PubMedPubMed CentralView ArticleGoogle Scholar
  24. Shuda M, Arora R, Kwun HJ, Feng H, Sarid R, Fernandez-Figueras MT, Tolstov Y, Gjoerup O, Mansukhani MM, Swerdlow SH, Chaudhary PM, Kirkwood JM, Nalesnik MA, Kant JA, Weiss LM, Moore PS, Chang Y: Human Merkel cell polyomavirus infection I. MCV T antigen expression in Merkel cell carcinoma, lymphoid tissues and lymphoid tumors. Int J Cancer. 2009, 125: 1243-1249. 10.1002/ijc.24510.PubMedView ArticleGoogle Scholar

Copyright

© Murakami et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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