Skip to main content

Advertisement

Immunization with HIV protease peptides linked to syngeneic erythrocytes

Article metrics

Abstract

New potent vaccine adjuvants are desirable for increasing the efficacy of novel vaccine modalities such as DNA and peptides. We therefore tested if syngeneic erythrocytes could serve as delivery vectors for selected HIV peptides and compared the potency of these constructs to immunization with peptides in phosphate buffered saline or in incomplete Freunds adjuvant.

Immunization of mice with peptides in a low dose (5 ng) coupled to erythrocytes induced a weak immune response in mice. These peptides alone (5 μg) gave no immune responses, while formulating the peptides (50 μg) in IFA induced strong homologous immunity as well as prominent cross reactivity to a related mutant epitope. Thus, vaccine delivery using syngeneic erythrocytes, although attractive for clinical use, might be of limited value due to the low amount of antigen that can be loaded per erythrocyte.

Findings

Peptide-based vaccines have been shown to be immunogenic in animal models, and well tolerated in man [1, 2]. One major benefit of peptide-based immunogens is the ease with which the vaccine can be modulated in order to fit new variants of a variable microbe such as HIV [3]. One application of such a vaccine strategy would be to target viral mutants conferring escape from antiretroviral drugs. As certain known mutations within epitopes of the HIV proteins are associated with resistance to the drugs [4] those epitopes variants could be included in a peptide-based vaccine. However these kinds of vaccines may need to be adjuvanted in order to be used in humans. The strategy of using syngeneic erythrocytes as delivery vectors is attractive, since no external compounds are used. Further, red blood cells (RBCs) are naturally removed by macrophages from the bloodstream and thereby targeted to immune cells. In addition, no toxic side effects have been found in host tissue following RBC-antigen delivery [5]. HIV Tat protein coupled to red blood cells was shown to induce as potent immune response as protein formulated in Freund's adjuvant [6]. The RBC method also induced protective immunity in mice and cats lethally challenged with HSV-1 and FIV-M2, respectively [7, 8]. The objective of the present study was to test the efficacy of syngeneic erythrocytes as delivery vectors and adjuvant for peptides deriving from the HIV-1 protease.

A human leukocyte antigen (HLA) A0201 restricted epitope, deriving from HIV-1 protease (PR75–84 wt, VLVGPTPVNI) and a mutant variant (PR75–84 d.mut) harboring two drug resistance mutations, V82F and I84V were resuspended in phosphate buffered saline (PBS), biotinylated and coupled to syngeneic erythrocytes by avidin-biotin bridges [5, 912]. The amount of peptide bound to the red blood cells was estimated to 25 ng/mL blood [7, 9]. Soluble peptides were formulated in PBS or in incomplete Freunds adjuvant (IFA) to a final concentration of 1 mg/ml. The peptide/IFA mixtures were sonicated (Sonica Vibra Cell VC100, Kenosia Ave, US) at a constant output of 40% for ~30–60 s on ice and the emulsions were used for immunization. C57Bl/6 mice, transgenic for the HLA-A0201 allele, were immunized according to Table 1. The animals were bled 10–12 days following each immunization and three mice per group were sacrificed 10 weeks after the last injection. IFN-γ ELISpot (Mabtech, Nacka, Sweden) was used for readout of cellular immunity. The plates were read by the AID ELISpot reader system. Statistical analysis was performed using GraphPad Prism 4.0. The nonparametric Kruskal-Wallis test was used to identify differences among the different groups, and Mann-Whitney U test was used for post hoc pair-wise comparisons.

Table 1 Immunization schedule of the study

A strong immune response was detected in blood against the individual peptides PR75–84 wt and PR75–84 d.mut following peptide/IFA-immunizations, Figures 1A and 1B. This response culminated 2 weeks after the second immunization and 10 weeks after the last injection the response was similar to that seen at week 7, Figures 1A and 1B. Of particular interest is, that immunization with either wild type or mutant epitope induced both high responses to the homologous peptide but also prominent cross-recognition to a related mutant peptide. Ten weeks after the last injection a response to both of the epitope variants could be seen in spleens from the PR75–84 wt immunized group, Figures 1C and 1D. The mice, in which RBC was used for peptide delivery showed, after repeated immunization with the RBC-HIV peptide conjugates (5–12,5 ng/injection), only a weak response in spleen cells, Figures 1C and 1D. This response was however significantly stronger (p = 0.0238) than that in mice immunized three times with a five hundred-fold higher concentration of soluble peptide (5 μg) formulated in PBS, Figures 1C and 1D.

Figure 1
figure1

IFN-γ response to PR 75–84 epitope variants. The kinetics of the IFN-γ secretion by peripheral blood mononuclear cells was investigated in response to (A) wild type peptide PR75–84 wt and (B) a mutant variant PR75–84 d.mut. Vaccination events are indicated by arrows. The response detected in spleens directed against the wild type epitope (C) and the mutant variant (D). Horizontal line indicates the geometric mean response. Blue open diamonds represent the response to peptide from a parallel experiment where mice were immunized three times with 5 μg of the peptide mixed in PBS. Immunizations were performed four weeks apart followed by a boost four months after the third immunization. The animals were bled 10–12 days after each injection.

We have previously shown that peptide immunization with the wild type protease epitope, PR75–84 wt, or the mutant variant, PR75–84 d.mut, both formulated in IFA, induces potent immune responses in mice [13]. This suggests that choosing either of these peptides as a component in a vaccine may suppress wild type virus as well as viral variants carrying drug-induced mutations [13]. Based on these findings we compared delivery of the two HIV protease peptides, PR75–84 wt and PR75–84 d.mut, either prepared in emulsion with IFA or coupled to syngeneic erythrocytes. To further increase the uptake of the peptide/erythrocyte complex by antigen presenting cells, the erythrocytes were chemically modified to be recognized as aging by macrophages and thereby cleared more rapidly by macrophages and antigen-presenting cells [5, 10]. A strong immune response was detected after peptide/IFA immunization, whereas only a weak cellular response was measurable upon four consecutive RBC-peptide conjugate immunization. In a parallel experiment, 5 μg of PR75–84 d.mut peptide in PBS was used for immunization and this amount of peptide without adjuvant did not induce any immune responses. These findings suggest that erythrocytes may serve as delivery vectors for very small amounts of peptide. Since the loading capacity of erythrocytes is limited, as well as the volume of blood for each injection, the enhanced presentation of peptides to macrophages by the modified erythrocytes is probably not sufficient to compensate for a small amount of peptide antigen in a clinical scenario.

References

  1. 1.

    Asjo B, Stavang H, Sorensen B, Baksaas I, Nyhus J, Langeland N: Phase I trial of a therapeutic HIV type 1 vaccine, Vacc-4x, in HIV type 1-infected individuals with or without antiretroviral therapy. AIDS Res Hum Retroviruses. 2002, 18: 1357-1365.

  2. 2.

    Pialoux G, Gahery-Segard H, Sermet S, Poncelet H, Fournier S, Gerard L, Tartar A, Gras-Masse H, Levy JP, Guillet JG: Lipopeptides induce cell-mediated anti-HIV immune responses in seronegative volunteers. Aids. 2001, 15: 1239-1249.

  3. 3.

    Sommerfelt MA, Nyhus J, Sorensen B: Novel peptide-based HIV-1 immunotherapy. Expert Opin Biol Ther. 2004, 4: 349-361.

  4. 4.

    Karlsson AC, Deeks SG, Barbour JD, Heiken BD, Younger SR, Hoh R, Lane M, Sallberg M, Ortiz GM, Demarest JF, et al: Dual Pressure from Antiretroviral Therapy and Cell-Mediated Immune Response on the Human Immunodeficiency Virus Type 1 Protease Gene. J Virol. 2003, 77: 6743-6752.

  5. 5.

    Chiarantini L: Vaccination Strategy Using Red Blood Cells as Antigen Delivery System. 2003, Landes Biosciences

  6. 6.

    Dominici S, Laguardia ME, Serafini G, Chiarantini L, Fortini C, Tripiciano A, Brocca-Cofano E, Scoglio A, Caputo A, Fiorelli V, et al: Red blood cell-mediated delivery of recombinant HIV-1 Tat protein in mice induces anti-Tat neutralizing antibodies and CTL. Vaccine. 2003, 21: 2073-2081.

  7. 7.

    Chiarantini L, Argnani R, Zucchini S, Stevanato L, Zabardi P, Grossi MP, Magnani M, Manservigi R: Red blood cells as delivery system for recombinant HSV-1 glycoprotein B: immunogenicity and protection in mice. Vaccine. 1997, 15: 276-280.

  8. 8.

    Chiarantini L, Matteucci D, Pistello M, Mancini U, Mazzetti P, Massi C, Giannecchini S, Lonetti I, Magnani M, Bendinelli M: AIDS vaccination studies using an ex vivo feline immunodeficiency virus model: homologous erythrocytes as a delivery system for preferential immunization with putative protective antigens. Clin Diagn Lab Immunol. 1998, 5: 235-241.

  9. 9.

    Magnani M, Chiarantini L, Mancini U: Preparation and characterization of biotinylated red blood cells. Biotechnol Appl Biochem. 1994, 20 (Pt 3): 335-345.

  10. 10.

    Chiarantini L, Droleskey R, Magnani M, DeLoach JR: In vitro targeting of erythrocytes to cytotoxic T-cells by coupling of Thy-1.2 monoclonal antibody. Biotechnol Appl Biochem. 1992, 15: 171-184.

  11. 11.

    Chiarantini L, Magnani M: Immobilization of enzymes and protein on red blood cells. 1996, Towata (NJ): Humana Press, 143-152.

  12. 12.

    Magnani M, Chiarantini L, Vittoria E, Mancini U, Rossi L, Fazi A: Red blood cells as an antigen-delivery system. Biotechnol Appl Biochem. 1992, 16: 188-194.

  13. 13.

    Boberg A, Sjostrand D, Rollman E, Hinkula J, Zuber B, Wahren B: Immunological cross-reactivity against a drug mutated HIV-1 protease epitope after DNA multi-CTL epitope construct immunization. Vaccine. 2006, 24: 4527-

Download references

Acknowledgements

This work was supported by grants from the AIDS Vaccine Integrated Project (AVIP), European Union and The Swedish Physicians Against AIDS Research Foundation, Sweden.

Author information

Correspondence to Andreas Boberg.

Additional information

Competing interests

The author(s) declare that they have no competing interests.

Authors' contributions

ABo contributed in the design of the study, immunization, read-out and analysis of the immunological data. He also performed the main writing. SD contributed in RBC-peptide linkage and analysis. Further contribution was writing and proof-reading of the manuscript. ABr and KH contributed with data analysis and proof-reading. JH contributed with animal expertise and proof-reading of the manuscript. MM and BW contributed with the project design and proof-reading.

Authors’ original submitted files for images

Below are the links to the authors’ original submitted files for images.

Authors’ original file for figure 1

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Keywords

  • Peptide
  • Delivery Vector
  • Strong Immune Response
  • Vaccine Adjuvant
  • Soluble Peptide