Fig. 1

Detection of EBV in poorly differentiated invasive ductal carcinoma tissue section using EBER-ISH nuclear staining. Epstein-Barr virus-encoded RNA-1 (EBER1) in situ hybridization technique was used on those samples which were relatively more positive by PCR (n = 14) and signal for viral transcripts was observed in 35.71% (5/14). Figure 1a shows a case of poorly differentiated invasive ductal carcinoma exhibiting nuclear positivity indicated by dark staining within malignant cells (arrows). (X 40 objective). Figure. 1b is the hematoxyline and eosin staining of the image in Fig. 1a . (X 40 objective). Figure 1c is a positive control from a case of EBV infected nasopharyngeal carcinoma tissue section showing dark nuclear staining (arrows). (X40 objective)