Bacteria flying under the radar: linking a bacterial infection to colon carcinogenesis
© Keenan and Frizelle; licensee BioMed Central Ltd. 2014
Received: 11 June 2014
Accepted: 18 August 2014
Published: 11 September 2014
The emergence of a link between Helicobacter pylori infection and an increased risk of gastric cancer has raised an awareness of a possible link between colonic microbiota and colorectal cancer. Pertubation of the colonic epithelium by toxin-producing strains of Bacteroides fragilis may increase the risk of premalignant transdifferentiation. However, like H. pylori, B. fragilis exhibit an ability to modulate the normal host response to infection. We speculate this may be an underappreciated risk factor in the genesis of colon carcinogenesis in individuals colonised with toxin-producing strains of B. fragilis.
Letters to the Editor
Gut bacteria and carcinogenesis
More than 90% of colorectal cancers are considered sporadic and being able to identify those who are at risk of developing this disease could reduce the number of colorectal cancer (CRC)-related deaths. Carcinogenesis is initiated when somatic changes induce irreversible DNA alterations that can persist indefinitely in cells and evidence is increasing of a role for bacterial species in this process, exemplified by chronic Helicobacter pylori infection and gastric cancer . The mechanism(s) of carcinogenesis associated with H. pylori colonization remains an area of debate but childhood acquisition of infection is reportedly associated with increased risk  that is strengthened by the carriage of strains carrying genes that code for specific virulence factors. The ca g-pathogenicity island is associated with the severity of precancerous lesions  via increased secretion of the pro-inflammatory cytokine interleukin (IL)-8 and the production of reactive oxygen species (ROS) . Strains that express the s1m1 variant of the VacA cytotoxin may increase risk via toxin-associated DNA damage . The expression of cytokines and chemokines (such as IL-8) create an immune microenvironment with the potential to exacerbate toxin- and/or ROS-mediated DNA damage . Thus, chronic inflammation may contribute to the hypermethylation of DNA that, in part, drives cells to become malignant.
Enterotoxigenic Bacteroides fragilis and CRC
Bacteroides fragilis is a ubiquitous anaerobic Gram-negative bacterium that colonises the human colon. Transmission is likely intra-familial and once acquired infection is persistent, much like H. pylori. Some strains produce a heat-labile toxin and infection with enterotoxigenic B. fragilis (ETBF) is associated with diarrhoea in children, adults and livestock. B. fragilis toxin (BFT) reportedly stimulates secretion of pro-inflammatory cytokine IL-8 from cultured intestinal epithelial cells  and therefore has the potential to provoke persistent, occult inflammation in vivo that, in turn, likely exacerbates the induction of colitis and tumour formation in ETBF-colonised Min mice . BFT also has the potential to perturb epithelial homeostasis through E-cadherin cleavage [9, 10] that results in β-catenin nuclear signalling and colonic epithelial proliferation . In mice, BFT expression is essential to disease pathogenesis . Intriguingly, H. pylori CagA protein reportedly has a similar effect on gastric epithelial cell homeostasis . Thus, like chronic H. pylori infection of the stomach, persistent ETBF infection may increase the risk of colon carcinogenesis via perturbation of apical-junctional complexes that increases the risk of premalignant transdifferentiation. This observation is supported by a recent Turkish study that found carriage of toxigenic, as opposed to non-toxigenic, B. fragilis increased in people with CRC .
Bacterial persistence as a risk factor for carcinogenesis?
H. pylori and B. fragilis share a common physiology that may contribute to their ability to provoke sustained, low-grade inflammation. Gram-negative bacteria are defined by their outer membrane that is implicated in the host response to infection. Generally, it is the lipid A moiety (or endotoxin) of outer membrane-associated lipopolyosaccharide that signals the presence of these bacteria in host tissues  and genes that encode the host sensory mechanism that recognise lipid A have been found in almost all studied vertebrates . However, the atypical structure of H. pylori lipid A  fails to activate the TLR4 signalling pathway and instead signals through TLR2 , making these bacteria less stimulatory to cells. B. fragilis lipid A shares the same structural differences as H. pylori lipid A . In addition, some B. fragilis strains express a polysaccharide antigen A (PSA) that also signals through TLR2 . These changes modulate the host response to infection, which fuels speculation that attenuated biological activity has the potential to contribute to their on-going infection of the human stomach and colon, respectively . Accordingly, we speculate that this inherent ability to “fly under the radar” of the normal host response to infection may be an underappreciated factor that contributes to the persistent colonisation considered as key to the increased risk of colon carcinogenesis in individuals infected with toxin-producing strains of B. fragilis.
- Blaser MJ, Atherton JC: Helicobacter pylori persistence: biology and disease. J Clin Invest. 2004, 113: 321-333. 10.1172/JCI20925.PubMedPubMed CentralView ArticleGoogle Scholar
- Blaser MJ, Chyou PH, Nomura A: Age at establishment of Helicobacter pylori infection and gastric carcinoma, gastric ulcer, and duodenal ulcer risk. Cancer Res. 1995, 55: 562-565.PubMedGoogle Scholar
- Plummer M, van Doorn LJ, Franceschi S, Kleter B, Canzian F, Vivas J, Lopez G, Colin D, Munoz N, Kato I: Helicobacter pylori cytotoxin-associated genotype and gastric precancerous lesions. J Natl Cancer Inst. 2007, 99: 1328-1334. 10.1093/jnci/djm120.PubMedView ArticleGoogle Scholar
- Naito Y, Yoshikawa T: Molecular and cellular mechanisms involved in Helicobacter pylori-induced inflammation and oxidative stress. Free Radic Biol Med. 2002, 33: 323-336. 10.1016/S0891-5849(02)00868-7.PubMedView ArticleGoogle Scholar
- Chitcholtan K, Hampton MB, Keenan JI: Outer membrane vesicles enhance the carcinogenic potential of Helicobacter pylori. Carcinogenesis. 2008, 29: 2400-2405. 10.1093/carcin/bgn218.PubMedView ArticleGoogle Scholar
- Mantovani A, Allavena P, Sica A, Balkwill F: Cancer-related inflammation. Nature. 2008, 454: 36-444.View ArticleGoogle Scholar
- Wu S, Powell J, Mathioudakis N, Kane S, Fernandez E, Sears CL: Bacteroides fragilis enterotoxin induces intestinal epithelial cell secretion of interleukin-8 through mitogen-activated protein kinases and a tyrosine kinase-regulated nuclear factor-kappaB pathway. Infect Immun. 2004, 72: 5832-5839. 10.1128/IAI.72.10.5832-5839.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Wu S, Rhee KJ, Albesiano E, Rabizadeh S, Wu X, Yen HR, Huso DL, Brancati FL, Wick E, McAllister F, Housseau F, Pardoll DM, Sears CL: A human colonic commensal promotes colon tumorigenesis via activation of T helper type 17 T cell responses. Nat Med. 2009, 15: 1016-1022. 10.1038/nm.2015.PubMedPubMed CentralView ArticleGoogle Scholar
- Wu S, Shin J, Zhang G, Cohen M, Franco A, Sears CL: The Bacteroides fragilis toxin binds to a specific intestinal epithelial cell receptor. Infect Immun. 2006, 74: 5382-5390. 10.1128/IAI.00060-06.PubMedPubMed CentralView ArticleGoogle Scholar
- Wu S, Lim KC, Huang J, Saidi RF, Sears CL: Bacteroides fragilis enterotoxin cleaves the zonula adherens protein, E-cadherin. Proc Natl Acad Sci U S A. 1998, 95: 14979-14984. 10.1073/pnas.95.25.14979.PubMedPubMed CentralView ArticleGoogle Scholar
- Wu S, Morin PJ, Maouyo D, Sears CL: Bacteroides fragilis enterotoxin induces c-Myc expression and cellular proliferation. Gastroenterology. 2003, 124: 392-400. 10.1053/gast.2003.50047.PubMedView ArticleGoogle Scholar
- Rhee KJ, Wu S, Wu X, Huso DL, Karim B, Franco AA, Rabizadeh S, Golub JE, Mathews LE, Shin J, Sartor RB, Golenbock D, Hamad AR, Gan CM, Housseau F, Sears CL: Induction of persistent colitis by a human commensal, enterotoxigenic Bacteroides fragilis, in wild-type C57BL/6 mice. Infect Immun. 2009, 77: 1708-1718. 10.1128/IAI.00814-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Murata-Kamiya N, Kurashima Y, Teishikata Y, Yamahashi Y, Saito Y, Higashi H, Aburatani H, Akiyama T, Peek RM, Azuma T, Hatakeyama M: Helicobacter pylori CagA interacts with E-cadherin and deregulates the beta-catenin signal that promotes intestinal transdifferentiation in gastric epithelial cells. Oncogene. 2007, 26: 4617-4626. 10.1038/sj.onc.1210251.PubMedView ArticleGoogle Scholar
- Toprak NU, Yagci A, Gulluoglu BM, Akin ML, Demirkalem P, Celenk T, Soyletir G: A possible role of Bacteroides fragilis enterotoxin in the aetiology of colorectal cancer. Clin Microbiol Infect. 2006, 12: 782-786.PubMedView ArticleGoogle Scholar
- Rietschel ET, Brade L, Brandenburg K, Flad HD, de Jong-Leuveninck J, Kawahara K, Lindner B, Loppnow H, Lüderitz T, Schade U, Seydel U, Zidorczyk S, Tacken A, Zähringer U, Brade H: Chemical structure and biologic activity of bacterial and synthetic lipid A. Rev Infect Dis. 1987, 9 (Suppl 5): S527-S536.PubMedGoogle Scholar
- Roach JC, Glusman G, Rowen L, Kaur A, Purcell MK, Smith KD, Hood LE, Aderem A: The evolution of vertebrate Toll-like receptors. Proc Natl Acad Sci U S A. 2005, 102: 9577-9582. 10.1073/pnas.0502272102.PubMedPubMed CentralView ArticleGoogle Scholar
- Moran AP, Helander IM, Kosunen TU: Compositional analysis of Helicobacter pylori rough-form lipopolysaccharides. J Bacteriol. 1992, 174: 1370-1377.PubMedPubMed CentralGoogle Scholar
- Smith MF, Mitchell A, Li G, Ding S, Fitzmaurice AM, Ryan K, Crowe S, Goldberg JB: Toll-like receptor (TLR) 2 and TLR5, but not TLR4, are required for Helicobacter pylori-induced NF-kappa B activation and chemokine expression by epithelial cells. J Biol Chem. 2003, 278: 32552-32560. 10.1074/jbc.M305536200.PubMedView ArticleGoogle Scholar
- Weintraub A, Zahringer U, Wollenweber HW, Seydel U, Rietschel ET: Structural characterization of the lipid A component of Bacteroides fragilis strain NCTC 9343 lipopolysaccharide. Eur J Biochem. 1989, 183: 425-431. 10.1111/j.1432-1033.1989.tb14945.x.PubMedView ArticleGoogle Scholar
- Round JL, Lee SM, Li J, Tran G, Jabri B, Chatila TA, Mazmanian SK: The Toll-like receptor 2 pathway establishes colonization by a commensal of the human microbiota. Science. 2011, 332: 974-977. 10.1126/science.1206095.PubMedPubMed CentralView ArticleGoogle Scholar
- Blaser MJ, Kirschner D: The equilibria that allow bacterial persistence in human hosts. Nature. 2007, 449: 843-849. 10.1038/nature06198.PubMedView ArticleGoogle Scholar
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