Figure 3

Above, FISH assay shows the MYC locus rearrangement with a break-apart probe (Vysis LSI MYC Dual Color Break Apart Rearrangement Probe Kit). A. MYC rearrangement-positive tumor cells, showing one yellow fusion signal, one orange and one green signals; B. Absence of MYC translocation, showing fusion signal patterns; Below, FISH assay shows the IGH/MYC/CEP8 Tri-Color Dual Fusion probe (Vysis IGH/MYC/CEP 8 Tri-Color DF FISH Probe Kit): C. The reciprocal t(8;14) in tumor cells showed a pattern of one orange, one green, two orange/green fusions, and two aqua centromeric signals; D. Absence of reciprocal translocation showed the two aqua, two orange, and two green signals pattern.