Table 1 Primer pairs used to amplify MCPyV LT-Ag sequence
Name | Sequence |
|---|---|
137 F-1122R | Forward 5’ GTGAGGTAGCTCATTTGCTCC 3’ Reverse 5’ CGGGAGGTATATCGGGTCCT 3’ |
137 F -569R | Forward 5’ GTGAGGTAGCTCATTTGCTCC 3’ Reverse 5’ACAATGCTGGCGAGACAACT 3’ |
615 F– 1538R | Forward 5’ CTGTCTGACGTGGGGAGAGTG 3’ Reverse 5’TTTTGGCTTTGGTGGAGTGC 3’ |
615 F– 1122R | Forward 5’ CTGTCTGACGTGGGGAGAGTG 3’ Reverse 5’ CGGGAGGTATATCGGGTCCT 3’ |
1101 F– 1538R | Forward 5’AGAGGACCCGATATACCTCCC 3’ Reverse 5’ TTTTGGCTTTGGTGGAGTGC 3’ |
1290 F– 2085R | Forward 5’ GAGCCTCCCTCGTCCTCTGAGG 3’ Reverse 5’CCTTGTGAGGTTTGAGGCGAGAT 3’ |
1482 F– 2085R | Forward 5’ AGCTCTGCAAGCTCTGCTAGT 3’ Reverse 5’ CCTTGTGAGGTTTGAGGCGAGAT 3’ |
1793 F– 2209R | Forward 5’ TACCTTCTGCACTATAAGCTTT 3’ Reverse 5’CTGGTCATTTCCAGCATCTCT 3’ |
1935 F– 2652R | Forward 5’ GAGGCCAGCTGTAATTGGAATT 3’ Reverse 5’AGCTTACAGCTACAGCACCG 3’ |
1935 F– 2518R | Forward 5’ GAGGCCAGCTGTAATTGGAATT 3’ Reverse 5’GCACACCCTAGTTCAAAAGGC 3’ |
2577 F– 3003R | Forward 5’ GATCTGCAACCAGGGCAAGGAAT 3’ Reverse 5’GAGGGTCCTGACCAGCTTCTA 3’ |
2577 F– 3204R | Forward 5’ GATCTGCAACCAGGGCAAGGAAT 3’ Reverse 5’AGGACCTCAAAAGGCCTCTCA 3’ |