Fig. 5

Ultrastructural analyses of nelfinavir treated 5r219 cells. 5r219 cells were induced with doxycycline and then exposed to 20 µM nelfinavir for 72 h. Cells were then processed for thin section and imaged by TEM. In the no drug controls (A), capsid structures were evident in the nucleus. Some contained an electron dense DNA core (white arrow) whereas others had an internal scaffold core (black arrow). In the cytoplasm and at the cell membrane, enveloped virions were evident (white arrowhead). In the nelfinavir treated cells (B), mature capsids were not observed however, within the nucleus, large dense aggregations were evident (white arrowheads). Scale bar = 1000 micron. c– cytoplasm, n– nucleus