Fig. 4

Nelfinavir induces gene expression of CHOP and Trb3. 5r219 cell cultures were induced with doxycycline and then exposed to 1 mM of nelfinavir for 5 min or 20 µM nelfinavir for 72 h or no drug (ND). At 24, 48 and 72 h after induction, RNAs were extracted from replicate cultures and were analyzed using RT qPCR methods (A). 5r219 cultures were induced with doxycycline and exposed to nelfinavir (1 mM for 5 min or 5 and 20 µM of drug for 72 h). The cells were harvested at 72 h post-induction and the proteins were analyzed by western blot methods (B). Molecular weight standards (kD) are shown in the left lane. Un: uninduced, Ind ND: Induced with doxycycline no drug control. The induced CHOP polypeptide is indicated by a line on the right of the panel