Fig. 3

High-dose short-duration exposure of nelfinavir and KSHV inhibition. 5r219 cells were lytically induced with doxycycline. After 1 h induction, the cells in replicates were treated with 1 mM nelfinavir mesylate (NFV) for 1 or 5 min. The drug was removed and the cells were incubated in medium containing only doxycycline. The cell culture supernatants were harvested at 72 h post-induction and viral genomes quantified using qPCR assays (A). Virus was also concentrated and the pelleted virus used to infect Vero cells. Fluorescence was examined after 48 h (B). Cell viability was examined for similarly treated cultures and compared to cells incubated with 20 µM nelfinavir continuously for 72 h (C)