Fig. 4

EPRS1 modulated proline-rich protein expression. A, Heat map of abundance changes in cells treated with 300 nmol/L halofuginone for 24 h compared to vehicle control (average of n = 2). Red represents upregulated proteins and green represents downregulated proteins. B–D, Gene Ontology enrichment of downregulated proteins, including biological process (B), cellular component (C), and molecular function (D). E, KEGG pathway enrichment of downregulated proteins. F, Gene set enrichment analysis showing significant enrichment of the indicated gene set comparing the inhibitor group with the DMSO control group. FDR, false discovery rate. G, Differential protein interaction analysis using the STRING database. Different colors represent the differential expression of proteins (red, upregulated proteins; green, downregulated proteins). The top 50 proteins with the closest interaction were screened and mapped in a protein interaction network. H, LAMC1 level in Hep3B cells treated with 300 nmol/L halofuginone for 24 h was determined by Western blot. I, Correlation between EPRS1 expression and downregulated proteins represented by LAMC1 and CCNB1 in TCGA-LIHC and GSE14520 datasets. P, proline