BK and JC polyomaviruses and risk of urothelial bladder carcinoma: a preliminary study in the northern shores of Persian Gulf, Iran

Table 1 Sequences of primers for detection of BK polyomavirus (BKPyV), John Cunningham polyomavirus (JCPyV) and internal controls

Virus	Primers name	Sequences of primers 5′ → 3′	Gene	Region in genome	Annealing temperature (°C)	Size	References
BKPyV and JCPyV	PEP-1F	AAGTCTTTAGGGTCTTCTACC	Large T antigen	4497–4518	56	276 bp	[14]
	DP-R1	CAGRGATCTAAAGCTTTAAGG		4764–4743
	PEP-1F	AAGTCTTTAGGGTCTTCTACC		4497–4518	56	176 bp
	PEP-R2	GGTGCCAACCTATGGAACAGA		4673–4652
BKPyV and JCPyV	BKJC-f	TGTACGGGACTGTAACACC	VP1	1629–1648	57	562 bp	[15]
	PoE2as	TCTGGGTACTTTGTYCTGTA		2191–2171
	PoE1s	GGAGGAGTAGAAGTTCTAGAA		1758–1779	55	433 bp
	PoE2as	TCTGGGTACTTTGTYCTGTA		2191–2171
Inernal controls	PCO3F	ACACAACTGTGTTCACTAGC	β globin	5,248,179	55	110 bp	[12]
	PCO4R	CAACTTCATCCACGTTCACC		5,248,288
	KRAS-F	GGTGAGTTTGTATTAAAAGGTACTGG	KRAS	10,423	55	263 bp	[13]
	KRAS-R	TCCTGCACCAGTAATATGCA		10,666

ISSN: 1750-9378