Fig. 2

HPV58 read fractions and the percentage of off-target human reads vary by archive type. A Box-plots showing the read fractions of HPV16, HPV18, HPV58, and HPV45 in archived DNA, frozen tissue, and FFPE tissue samples. Although read fractions tended to be lower for HPV58 than for HPV16 and HPV18, HPV58 was detected more frequently (especially at read fractions between 20 and 80%) in FFPE tissue samples than in archived DNA and frozen tissue samples. B Associations between HPV16, HPV18, HPV58, and HPV45 read fractions and the percentages of off-target human reads in all samples. For HPV16 and HPV18, the HPV genotype-specific read fractions tended to be lower in samples with higher percentages of off-target human reads. In contrast, the frequency of HPV58 reads tended to be higher in frozen and FFPE tissue samples with higher percentages of human reads