Fig. 3

Establishment of a mouse tumorigenesis model and detection of related gene expression. A The HPV16 E6 and HPV16 E7 genes in the tissues of the TC-1 transplantation tumor group were detected by RT-PCR (HPV16 E6 249 bp, HPV16 E7 199 bp). B HPV18 E6E7 fusion gene expression in the tumor tissues of the three groups was detected by RT-PCR. M: DNA ladder (100 bp) A: U14/LV-HPV18 E6E7 group (HPV18 E6E7 789 bp), B: U14/LV-NC group, C: U14 group. C HPV58 E6E7 fusion gene expression in the tumor tissues of the three groups was detected by RT-PCR. M: DNA marker a1, a2, a3: U14/LV-HPV58 E6E7 group (HPV58 E6E7 741 bp), b1, b2, b3: U14/LV-NC group, c1, c2, C3: U14 group. D Western blot results of the HPV16 E6 and E7 proteins in the tissues of the TC-1 transplantation tumors. E Western blot results of the HPV18 E6 and HPV18 E7 proteins in three groups of mouse tumor tissues: A: U14/LV-HPV18 E6E7 group, B: U14/LV-NC group, C: U14 group. F Western blot results of the HPV58 E6E7 + His-tag fusion protein in three groups of tumor tissues: a1, a2, a3: U14/LV-HPV58 E6E7 group, b1, b2, b3: U14/LV-NC group, c1, c2 c3: U14 group