Fig. 8

In vivo experiments prove that ZFAS1 can regulate tumor and autophagy. A HONE-1 cells stably expressing sh-ZFAS1 or hybrid control were transplanted into the axilla of nude mice to establish a tumor growth model. Representative image of the formed tumor (A), tumor volume growth curve (B) and weight (C). D HE and Ki67 staining in the tumors of each treatment group. The arrow points to the area of tumor necrosis. E After transfection of sh-ZFAS1, qRT-PCR was used to detect the expression of miR-100-3p and ATG10. F Expression of EMT related genes. G QRT-PCR detects the expression of autophagy-related genes (LC3, Beclin1, p62) in different treatment groups. H WB detected the expression of PI3K/AKT signaling pathway in different treatment groups. *p < 0.05, compared with sh-NC group. Sh-NC: short hairpin RNA contrl, sh-ZFAS1: short hairpin RNA ZFAS1