Fig. 5

ZFAS1 exerts a cancer-promoting effect by targeting miR-100-3p. A Use the bioinformatics tools in lncATLAS to predict the subcellular localization of ZFAS1. B QRT-PCR analysis of subcellular ZFAS1 expression in the nucleus and cytoplasm of HONE-1 cells. U6 and 18S were used as endogenous controls. C QRT-PCR was used to detect the expression levels of miR-100-3p, miR-302a-3p and miR-512-3p in HONE-1 and HK-1 cells. D The expression of miR-100-3p in clinical samples, n = 53. E Pearson correlation was used to detect the correlation between the expression of miR-100-3p and ZFAS1. F Dual luciferase report verifies the targeting of ZFAS1 and miR-100-3p. The binding site prediction comes from the lncbase database. G RIP assay verifies the targeting of ZFAS1 and miR-100-3p. H The expression of miR-100-3p in different treatment groups in HONE-1 and HK-1 cells. I CCK8 detects the cell viability of each treatment group. J Transwell detected the migration of NPC in each treatment group. K Detection of NPC cell apoptosis in different treatment groups. L Expression of EMT related genes and proteins. *p < 0.05, compared with NC group, #p < 0.05, compared with mimics group. NC: normal control, oe-ZFAS1: over-expression ZFAS1, si-NC: Small interfering RNA NC, si-ZFAS1: Small interfering RNA ZFAS1