Fig. 4

Induction of cross-neutralizing antibodies in immunized mice against HCVcc. Mice sera collected on day 0 and 56 were mixed at a 1:100 dilution with JFH-1/HCVcc and incubated for 1 h at 37 °C and subsequently applied to Huh-7 cells for 6 h in triplicate. Pre-immune sera was used as a negative control. The data are shown as the percent neutralization of JFH-1/HCVcc from sera of three mice groups (each had 8 mice) with different immunizations relative to the controls