Fig. 3

Cytokine levels in vaccinated mice groups. Cytokine production by splenocytes were evaluated 2 weeks after the last immunization. Splenocytes were cultured in duplicate and stimulated in vitro with HCV E2 protein (at 10 µg/ml), ConA as positive control (not shown) or medium alone (medium). IFN-γ (a) and IL-4 (b) levels in cell supernatants were measured by ELISA. The data are presented as the mean levels of cytokine ± standard deviation for 6 immunized mice in each group. One-way ANOVA was used for statistical analysis (P < 0.05 denoted as *, P < 0.01 denoted as **, P < 0.0001 denoted as **** and ns denoted as non-significant)