Table 2 Methylation oligonucleotide sequences and conditions for MSP
From: Host proteome linked to HPV E7-mediated specific gene hypermethylation in cancer pathways
Oligonucleotides | Sequence (5’–3’) | Amplicon (bp) | Tm (°C) |
|---|---|---|---|
C1QBP | FW: TAGCGGATTCGGTAGCGTAG | 152 | 54 |
RW: CCTAAAACGCCGCCGAAAAC | |||
CDKN2A | FW: TTCGAGTATTCGTTTACGGCGT | 159 | 54 |
RW: AACGCACGCGATCCGCC | |||
BCAP31 | FW: TTCGGTTTTCGGTCGCGGTAT | 124 | 55 |
RW: CCAACCCGACGCGCGAAA | |||
PTMS | FW: CGTTTTATTTTTTTTTCGCGGTCG | 128 | 54 |
RW: CGACGCTACCCGACGAAAAA |