Investigation of CTNNB1 gene mutations and expression in hepatocellular carcinoma and cirrhosis in association with hepatitis B virus infection

Table 2 The sequence of primers used for amplification of CTNNB1 gene, HBsAg coding region and gene expression analysis

Gene	Forward 5′- 3′	Revers 5′- 3′	PCR size	Ta_a	Ref
β-catenin	CGTGGACAATGGCTACTCAA	CACTCAGAGAAGGAGCTGTGG	150	57	^b
c-Myc	GGACGACGAGACCTTCATCAA	CCAGCTTCTCTGAGACGAGCTT	92	60	[10]
HMBS	CCCTGCCAGAGAAGAGTGTG	GTGTTGAGGTTTCCCCGAAT	109	57	^b
GAPDH	CGACCACTTTGTCAAGCTCA	AGGGGTCTACATGGCAACTG	228	58	[17]
HBS1	GAGTCTAGACTCGTGGTGGACTTC	AAATKGCACTAGTAAACTGAGCCA	448	58	[18]
HBS2	CGTGGTGGACTTCTCTCA ATTTTC	GCCARGAGAAACGGRCTGAGGCCC	417	60	[18]
CTNNB1 ex3–5	TAGCTGATTTGATGGAGTTGG	CTCACGATGATGGGAAAGGT	994	57	^b
CTNNB1 ex3, outer	TGCTTTTCTTGGCTGTCTTTC	CCTAAATGGTAAAAGTGACATTGC	500	55	^b
CTNNB1 ex3, inner	TGCTAATACTGTTTCGTATTTATAGC	TTCTGACTTTCAGTAAGGCAATG	293	53	^b

ISSN: 1750-9378