Fig. 2
Confirmation of tumor-specific expression of fish TTRgrEEN genes. Expression of zebrafish TTRgrEEN genes in cDNA from zebrafish tumor and normal liver tissues. T1 - hepatocellular carcinoma, T2 - spermatocytic seminoma, N1, N2 - pooled normal liver. 1 – camk4 – Danio rerio calcium/calmodulin-dependent protein kinase IV. 2 – fus – Danio rerio FUS RNA binding protein. 3 – ssbp3a – Danio rerio single stranded DNA binding protein 3a. 4 – ripply1 – Danio rerio ripply transcriptional repressor 1. 5 – tgfbrb2b – Danio rerio transforming growth factor beta receptor 2b. 6 – lepa – Danio rerio leptin a. 7 – sobpa – Danio rerio sine oculis binding protein homolog (Drosophila) a. 8 – ccdc40 – Danio rerio coiled-coil domain containing 40. 9 – sema7a – Danio rerio semaphorin 7A, transcript variant 2. 10 – ephb3a – Danio rerio eph receptor B3a. 11 – spry1 – Danio rerio sprouty homolog 1, antagonist of FGF signaling (Drosophila). 12 – lmx1b – Danio rerio LIM homeobox transcription factor 1, beta b. 13 – nr2e1 – Danio rerio nuclear receptor subfamily 2, group E, member 1. 14 – cacna1da– Danio rerio calcium channel, voltage-dependent, L type, alpha 1D subunit, a. NC – no template control, PC – positive control – gapdh – Danio rerio