Table 2 Nucleotide sequences of primers used for real-time RT-PCR
From: Evaluating the expression level of HERV-K env, np9, rec and gag in breast tissue
Gene | Forward primer (5ā²-3ā²) | Reverse primer (5ā²-3ā²) |
|---|---|---|
GAPDH | ATGTTCGTCATGGGTGTGAA | GGTGCTAAGCAGTTGGTGGT |
rec | ATCGAGCACCGTTGACTCACAAGA | GGTACACCTGCAGACACCATTGAT |
np9 | AGATGTCTGCAGGTGTACCCA | CTCTTGCTTTTCCCCACATTTC |
env | TAACCCTGTCACTTGGATT | ATGTCACTGTCTCTTCGG |
gag | AGCAGGTCAGGTGACCGTAAC | GGTGCCATAGCATTGTCTCCT |