Fig. 3

Scatter graph of Q- Real time PCR. a, b show Standard curve for BKV rt-QPCR. BKV plasmid DNA, in serial dilutions ranging from 10,000 to 10,000,000 copies/ml, has been amplified by rt-Q PCR. Fluorescence intensity was plotted against cycle number. c, d describe the distribution of BKV loads expressed as DNA copies per milliliter. Short lines indicate medians and ranges of the viral load in the samples. The highest viral load was 27,421 while the lowest viral load of 92 copies/ml. e, f combined scatter diagram between the samples curve and the standard curve. The Linear range of the assay obtained by use of known serial dilutions of the BKV plasmid as a template. The BKV copies number per samples were calculated from the standard curve