Fig. 4

Anti-B7-H4 antibody upregulates apoptotic proteins and downregulates mitogenic signaling. EBV-infected Pfeiffer cells were cultured with or without the Z-DEVD-fmk (caspase 3 inhibitor) or Z-LEHD-fmk (caspase 9 inhibitor) for 2 h before stimulation with anti-B7-H4 antibodies (5 μg/ml) for 3 days. Cellular proteins were observed by Western blotting after 72 h with antibodies of Bcl-2, p-Bcl-2, Bax, caspase 3, caspase 9, p-Akt, Akt, p-Erk1/2, Erk1/2 and GAPDH