Table 1 Primers used in this study
From: High-risk type human papillomavirus infection and p16 expression in laryngeal cancer
PCR primer | Sequence (5′–3′) |
|---|---|
GP5+ | TTTGTTACTGTGGTAGATACTAC |
GP6+ | GAAAAATAAACTGTAAATCATATTC |
MY09 | CGTCCMARRGGAWACTGATC |
MY11 | GCMCAGGGWCATAAYAATGG |
PC04 | CAACTTCATCCACGTTCACC |
GH20 | GAAGAGCCAAGGACAGGTAC |
Real-time PCR primer and TaqMan probe | Sequence (5′–3′) |
E2-F | AACGAAGTATCCTCTCCTGAAATTATTAG |
E2-R | CCAAGGCGACGGCTTTG |
E2-Probe | FAM-CACCCCGCCGCGACCCATA-TAMRA |
E6-F | GAGAACTGCAATGTTTCAGGACC |
E6-R | TGTATAGTTGTTTGCAGCTCTGTGC |
E6-Probe | FAM-CAGGAGCGACCCAGAAAGTTACCACAGTT-TAMRA |
β-globin-F | TGGGTTTCTGATAGGCACTGACT |
β-globin-R | AACAGCATCAGGAGTGGACAGAT |
β-globin-Probe | FAM-TCTACCCTTGGACCCAGAGGTTCTTTGAGT-TAMRA |
β-actin-F | GCGAGAAGATGACCCAGATC |
β-actin-R | CCAGTGGTACGGCCAGAGG |
β-actin-Probe | FAM-CCAGCCATGTACGTTGCTATCCAGGC-TAMRA |