Fig. 3

Restoration of miRNA expression after RNA interference study. a Relative expression of miR-122 in HepG2 cells by co-transfection with HBx plasmid (0.8 μg) and HBx mRNA specific siRNA (40 p mole) or Negative Control. b Relative expressions of miR-122 was measured when HepG2 cells were co-transfected with 1.3 fold full length HBV genome (0.8 μg) and HBx mRNA specific siRNA (40 p mole) or Negative Control. c HepG2.2.15 cells were transfected with HBx siRNA (80 p mole) or Negative Control and relative expressions of miR-122 was measured. Data plotted are the mean ± SD normalized to RNU6 expression. The experiments were performed in triplicate (*P < 0.05, **P < 0.01, ***P < 0.001; Student’s t-test). RNA were collected 48 h after each transfection