Fig. 2
From: miR-23b as a potential tumor suppressor and its regulation by DNA methylation in cervical cancer
miR-23b is deregulated by methylation of its promoter region in cervical cancer cell lines. a The analysis of the methylation status revealed the almost 100 % of total input DNA had methylated miR-23b gene promoter in C33A, HeLa, SiHa and CaSki cells untreated with 5′-Aza-CdR. b The relative expression levels of miR-23b were decreased in C33A, HeLa and CaSki cells compared to the levels observed in SiHa cells. c Treatment with 10 μM 5′-Aza-CdR significantly increased the expression of miR-23b in the four cell lines (weft bars) compared to untreated cells (black bars). The increased relative expression of miR-23b was ≥50 % over the initial expression (W/T) in C33A, HeLa and CaSki but not in SiHa cells. *Statistically significant difference (p < 0.05); W/T: Untreated cells