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Table 1 PCR steps. Cycle's time temp PCR steps

From: Frequency of Human Papilloma Virus (HPV) subtypes 31,33,35,39 and 45 among Yemeni women with cervical cancer

Step

Temperature °C

Time

Cycles

1

95 °C

Pause

2

95 °C

15 min

1

3

95 °C

30 s

42

63 °C

30 s

72 °C

40 s

4

72 °C

1 min

1

5

10 °C

Storage

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Infectious Agents and Cancer

ISSN: 1750-9378

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