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Table 1 PCR steps. Cycle's time temp PCR steps

From: Frequency of Human Papilloma Virus (HPV) subtypes 31,33,35,39 and 45 among Yemeni women with cervical cancer

Step Temperature °C Time Cycles
1 95 °C Pause
2 95 °C 15 min 1
3 95 °C 30 s 42
63 °C 30 s
72 °C 40 s
4 72 °C 1 min 1
5 10 °C Storage