Table 1 Primers used in this study
Primer | Relevant sequence | Size of amplicon | Amplification cycles | Annealing temperature | References |
|---|---|---|---|---|---|
flgE- F | 5′ CCGATCAAATCCTTAACACC 3′ | 381 | 30 | 52 | This study |
flgE- R | 3′ AGGCTTAAAAACATGCGAAC 5′ |  |  |  |  |
flhA- F | 5′ TCATTGGAGGGTTTTTAGTGG 3′ | 155 | 26 | 60 | [23] |
flhA- R | 3′ GGTGCGAGTGGCGACAAT 5′ |  |  |  |  |
flaA- F | 5′ TAGACACCACCAACGCTAAA 3′ | 239 | 28 | 62 | This study |
flaA- R | 3′ TGCATTCTAGGGGGTTGTAT 5′ |  |  |  |  |
flaB- F | 5′ GTCAATGGCGTGAATGATTA 3′ | 213 | 30 | 60 | This study |
flaB- R | 3′ ATTCACGGTCCCAATTTCTA 5′ |  |  |  |  |
babA- F | 5′ ATCGATCCACTTCCATCACT 3′ | 292 | 40 | 48 | This study |
babA- R | 3′ GTTACGCTTTTGCCGTCTAT 5′ |  |  |  |  |
hopZ- F | 5′ GCGCCGTTACTAGCATGATCA 3′ | 101 | 24 | 60 | [24] |
hopZ- R | 3′ GAAATCTTTCGGCGCGTTT 5′ |  |  |  |  |
sabA- F | 5′ AAAGCATTCAAAACGCCAAC 3′ | 163 | 24 | 60 | This study |
sabA- R | 3′ CCCGCATAAAGACTCCAAAA 5′ |  |  |  |  |
alpA- F | 5′ GCACGATCGGTAGCCAGACT 3′ | 90 | 22 | 60 | [24] |
alpA- R | 3′ ACACATTCCCCGCATTCAAG 5′ |  |  |  |  |
alpB- F | 5′ ACGCTAAGAAACAGCCCTCAAC 3′ | 82 | 26 | 60 | [24] |
alpB- R | 3′ TCATGCGTAACCCCACATCA 5′ |  |  |  |  |
hpaA- F | 5′ GAGCGTGGTGGCTTTGTTAGT 3′ | 90 | 26 | 60 | [24] |
hpaA- R | 3′ TCGCTAGCTGGATGGTAATTCA 3′ |  |  |  |  |
rpoB- F | 5′ TTTAGGTAAGCGCGTGGATT 3′ | 301 | 27 | 59 | This study |
rpoB- R | 3′ AATCAGCTTTGGATGGAACG 5′ |  |  |  |  |