Table 4 Primer sequences for microRNA methylation analysis
microRNA | Primer sequence |
|---|---|
hsa-miR148a FORWARD (BSP) | 5’-TGGGTATTTGTTTTTGTTGATTG-3’ |
hsa-miR148a REVERSE (BSP) | 5’ACTACACTTAAACCCCCTCTAACC-3’ |
hsa-miR152 FORWARD (BSP) | 5’-GGAATTTTGTGTTATTTTTGATTG-3’ |
hsa-miR152 REVERSE (BSP) | 5’CCAAACAAATATTCCACTACAAAC-3’ |
hsa-miR148a U-FORWARD (MSP) | 5’TTGATGTTGTTAGTGTTTTAGATGT-3’ |
hsa-miR148a U-REVERSE (MSP) | 5’AAAAAAATTAATCAAATTCCTCATA-3’ |
hsa-miR148a M-FORWARD (MSP) | 5’GTCGATGTCGTTAGTGTTTTAGAC-3’ |
hsa-miR148a M-REVERSE (MSP) | 5’AAAAAAATTAATCAAATTCCTCGTA-3’ |