Figure 3
From: Human Mammary Tumor Virus (HMTV) sequences in human milk
Detection of HMTV- env /LTR junction sequences in P2 RNA. cDNA synthesized from high speed pellet (P2) RNA using primer LTR3 and PCR performed using primers ENV-3LR’, LTR-5MR and semi nested PCR with ENV-3LR’ and LTR-1R’ as described in Methods. A. Diagram of HMTV viral RNA structure and location of the primers. B. Sequence amplified from cDNA using primers ENV-3LR’, LTR-5MR by PCR and by semi nested PCR with ENV-3LR’ and LTR-1R’ primers (GenBank KJ831811). C. 1% agarose gel electrophoresis of HMTV-env/LTR 1018 bp amplicon. Lane 1: HMTV-env/LTR positive cDNA sample. Lane 2: 1 kb plus DNA ladder. Lane 3: Southern blot hybridization using 32P end labeled probe for HMTV-env/LTR positive cDNA sample.