Figure 7
No detectable HV-68 RNA transcripts in primary mammary tumors and secondary metastases. At death, or at day 44 following tumor cell transplantation, groups of mice (N = 10) were euthanized. Primary mammary tumors and secondary metastases were excised from each animal, and RNA extracted from each tissue. Quantitative RT-PCR was performed to detect the presence of one replicating HV-68 transcript (ORF65) and one latent HV-68 transcript (K3). Results from 5 different animals (1, 2, 3, 4, 5) are shown as amplified fragments electrophoresed on ethidium bromide stained polyacryamide gels. The correct size of each amplified fragment is indicated by comparison to DNA ladders run on the same gel (Ladder). Positive controls for the amplification of each gene were also included in each reaction (+). The presence of GAPDH RNA was used as a positive control in each RT-PCR reaction.