Figure 1
From: TP 53 and MDM2 gene polymorphisms and risk of hepatocellular carcinoma among Italian patients
PCR amplification followed by restriction fragment length polymorphism (PCR-RFLP) to determine MDM 2 SNP309 polymorphism. MDM 2 SNP309 T allele was not cleaved by Msp AI endonuclease and had a single band of 174 bp. The MDM 2 SNP309 G allele was cleaved by Msp AI and had two small fragments of 126 and 48 bp. The MDM 2 SNP309 heterozygote had three bands of 174, 126 and 48 bp.