- Poster presentation
- Open Access
Cervical and anal HPV infection and dysplasia in HIV-infected women
Infectious Agents and Cancer volume 4, Article number: P40 (2009)
HIV-infected women are at increased risk of cervical HPV infection and neoplasia. We investigated whether HIV-infected women are also at high risk for HPV infection of the anal canal and anal dysplasia.
We performed a pilot, prospective, observational study on 100 HIV-infected women presenting for routine HIV care. Subjects were interviewed and underwent cervical and anal testing with cytology and HPV with Hybrid Capture 2 assay (Digene Corporation, Gaithersburg, MD). High resolution anoscopy (HRA) or colposcopy were performed if indicated and subjects with high grade dysplasia were referred for treatment. Secondary analyses were performed to assess for associated risk factors for abnormal cervical or anal cytology or HPV infection such as CD4 T-cell count using SPSS for Windows, version 15.0.
The average age of the subjects was 41 years and 78 percent of the women were currently on highly active antiretroviral therapy.
The prevalence of high-risk HPV infection in the cervix was 24 percent and cervical cytological abnormality was 21.6 percent. Of the 24 patients with cervical HPV infection, 15 (62.5%) had cervical cytological abnormalities. (Fisher Exact Test, p = 0.0001).
The prevalence of high risk HPV infection in the anus was 16 percent and anal cytological abnormality was 17 percent. Of the 16 patients with anal HPV infection, 10 (62.5%) had anal cytological abnormalities (Fisher Exact Test, p = 0.0001).
Eleven of 24 women (46%) who had high risk HPV in the cervix also had high risk HPV detected in the anal canal (Fisher Exact Test, p = 0.0001). Eight of 21 women (38 percent) who had abnormal cervical cytology also had abnormal anal cytology (Fisher Exact Test, p = 0.0001).
Subjects with anal HPV infection (mean CD4 count 349 cells/μl) had significantly lower mean CD4 counts compared to subjects without anal HPV infection (mean CD4, 555 cells/μl) (t test, p = 0.007). Subjects with abnormal anal cytology (mean CD4 393 cells/μl) had significantly lower mean CD4 counts compared with subjects with normal anal cytology (mean CD4, 550 cells/μl) (t-test, p = 0.007).
Similarly, subjects with cervical HPV infection were significantly more likely to have lower mean CD4 count (394 cells/μl) compared to those without cervical HPV infection (564 cells/μl) (t-test, p = .01). Subjects with abnormal cervical cytology also had lower mean CD4 count (372 cells/μl) compared to women with normal cervical cytology (CD4, 549 cells/μl) (t-test, p = .004).
Of the 16 patients who underwent colposcopy, eight had low-grade and five had high-grade cervical or vaginal dysplasia. Of the 11 women who underwent HRA, six had low-grade and three had high-grade anal dysplasia.
Anal and cervical HPV infections are common in HIV-infected women. HIV-infected women have high rates of anal and cervical dysplasia. These findings provide further data for developing anal cytology screening recommendations for this patient population.