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Table 1 Ab levels to whole gp120 and to CD4bs in sera of mice immunized with envelope proteins that have or lack CD4 binding activitya

From: Antibodies to the CD4-binding site of HIV-1 gp120 suppress gp120-specific CD4 T cell response while enhancing antibody response

  Immunogen &
Adjuvant
n Anti-gp120
(end-point titer)
Anti-CD4bs
(ID50)d
Experiment 1b No Ag & IFA 2 < 20 < 20
  CD4bs+ Env & IFA 4 > 2500 310 (210–470)e
  CD4bs- Env & IFA 3 > 2500 < 20
Experiment 2c No Ag & RIBI 6 < 100 < 100
  CD4bs+ Env & RIBI 5 9060 (2500–12500) 2160 (630–5900)
  CD4bs- Env & RIBI 5 > 12500 (12500->12500) < 100
  1. aSera were collected two weeks after the final immunizations and tested for reactivity with recombinant gp120LAI in ELISA.
  2. bAll mice were B10.A, except for two BALB/c in the second group. Each animal was immunized 4x i.p. with 5 μg of antigen in IFA.
  3. cAll mice were BALB/c and each was immunized 5x i.p. with 5 μg of antigen in RIBI.
  4. dSerum dilutions that block CD4 binding to gp120LAI by 50%.
  5. eGeometric mean and range of the Ab levels from all mice in each group.