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Table 1 Results of Repeated Quantitative Real-time PCR on Cytobrush Specimens

From: Absolute quantitative real-time polymerase chain reaction for the measurement of human papillomavirus E7 mRNA in cervical cytobrush specimens

ID

HPV type assayed

mRNA Log Starting Quantity***

mRNA Copy Number****

  

Repeat 1

Repeat 2

Repeat 1

Repeat 2

1

16

1.3845

1.409

3.99

4.09

2

16

0

0

0

0

3

16

0.7095

1.026

2.03

2.79

4

16

0

0

0

0

5*

16

0

0

0

0

6

16

1.5385

1.274

4.66

3.58

7

16

1.4075

1.4615

4.09

4.31

8*

16

0

0

0

0

9**

16

0

0

0

0

10

16

1.5605

1.6785

4.76

5.36

11

16

0.737

0.8855

2.09

2.42

12

16

0

0.253

0

1.29

13

16

0.6945

0.656

2.00

1.93

14

16

0.662

0.5

1.94

1.65

15

16

1.35

1.4165

3.86

4.12

16

16

0.468

0.458

1.60

1.58

17

18

0.6625

1.0695

1.94

2.91

18

18

4.2175

4.1265

67.86

61.96

  1. Note: Coefficient of Variation (CV) = 10.4%
  2. *HPV16 and HPV18 negative, HPV consensus positive; used as specificity control
  3. **HPV16, HPV18, and HPV consensus negative; used as specificity control
  4. ***Log SQ = ln(copy number)
  5. ****per 20 ng cDNA