Figure 2
Standard Curve from HPV 16 E7 Assay. This figure represents the standard curve of the HPV 16 PCR. Note, the standard dilutions appear as blue circles on the graph, and the unknown samples appear as red squares. The calculated regression line of the curve is given above the graph. In this case, Y = -2.882X + 34.568. Setting Y equal to the CT for the unknown, solving for X will give the starting quantity for the unknown sample.