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Table 1 Triplex PCR primers for amplifying the target genes in E. coli O157:H7 and Vibrio cholerae O139

From: Detection and identification of enterohemorrhagic Escherichia coli O157:H7 and Vibrio cholerae O139 using oligonucleotide microarray

Target gene Target organism Sequence(5'-3') PCR product length (bp) Concentration in PCR mixture
Stx 1 E. coli O157 F: GAA TTT ACC TTA GAC TTC TCG AC 250 0.15 μmol/L
   R: TCC TGT TAA CAA ATC CTG TCA C   0.75 μmol/L
Stx 2 E. coli O157 F: TAC GAT AGA CTT TTC GAC TCA AC 207 0.1 μmol/L
   R: TCA ATA ATC AGA CGA AGA TGG TC   0.5 μmol/L
Uid A E. coli O157:H7 F: TAA TGA GGA GTC CCT TAT GTT AC 179 0.1 μmol/L
   R: ACT GAT CGT TAA AAC TGC CTG G   0.5 μmol/L
Ctx A Vibrio cholerae F: ACT CAG ACG GGA TTT GTT AGG C 304 0.15 μmol/L
   R: ATC TAT CTC TGT AGC CCC TAT TAC   0.75 μmol/L
Tcp A Vibrio cholerae F: TTG ACC CAA GCA CAA TGT AAG AC 241 0.15 μmol/L
   R: CTA CTG TGA ATG GAG CAG TTC C   0.75 μmol/L
LPS gt Vibrio cholerae F: ACA TCT GTA GGG ATT GTA TTG AC 340 0.2 μmol/L
  O139 R: ATA ACA ACT GAG ATA TCA AGC GTC   1.0 μmol/L