Table 1 Triplex PCR primers for amplifying the target genes in E. coli O157:H7 and Vibrio cholerae O139
Target gene | Target organism | Sequence(5'-3') | PCR product length (bp) | Concentration in PCR mixture |
|---|---|---|---|---|
Stx 1 | E. coli O157 | F: GAA TTT ACC TTA GAC TTC TCG AC | 250 | 0.15 μmol/L |
R: TCC TGT TAA CAA ATC CTG TCA C | 0.75 μmol/L | |||
Stx 2 | E. coli O157 | F: TAC GAT AGA CTT TTC GAC TCA AC | 207 | 0.1 μmol/L |
R: TCA ATA ATC AGA CGA AGA TGG TC | 0.5 μmol/L | |||
Uid A | E. coli O157:H7 | F: TAA TGA GGA GTC CCT TAT GTT AC | 179 | 0.1 μmol/L |
R: ACT GAT CGT TAA AAC TGC CTG G | 0.5 μmol/L | |||
Ctx A | Vibrio cholerae | F: ACT CAG ACG GGA TTT GTT AGG C | 304 | 0.15 μmol/L |
R: ATC TAT CTC TGT AGC CCC TAT TAC | 0.75 μmol/L | |||
Tcp A | Vibrio cholerae | F: TTG ACC CAA GCA CAA TGT AAG AC | 241 | 0.15 μmol/L |
R: CTA CTG TGA ATG GAG CAG TTC C | 0.75 μmol/L | |||
LPS gt | Vibrio cholerae | F: ACA TCT GTA GGG ATT GTA TTG AC | 340 | 0.2 μmol/L |
O139 | R: ATA ACA ACT GAG ATA TCA AGC GTC | 1.0 μmol/L |