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Table 1 HPV genotyping by DNA Sequencing v. Digene HC2 Test

From: Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories

PCR/DNA Sequencing Test Results by Digene HC2
Type Positive Cases Prevalence (%) High-risk+ Negative
16 29 (27.2) 11 18
56 9 (8.5) 9 0
31 7 (6.5) 5 2
6 6 (5.6) 2 4
18 6 (5.6) 6 0
54 6 (5.6) 3 3
58 6 (5.6) 5 1
66 6 (5.6) 6 0
59 4 (3.7) 4 0
45 3 (2.8) 2 1
83 3 (2.8) 2 1
32 2 (1.9) 0 2
35 2 (1.9) 1 1
39 2 (1.9) 2 0
40 2 (1.9) 0 2
52 2 (1.9) 1 1
33 1 (0.9) 1 0
53 1 (0.9) 1 0
62 1 (0.9) 1 0
68 1 (0.9) 1 0
70 1 (0.9) 1 0
72 1 (0.9) 0 1
73 1 (0.9) 0 1
M16 1 (0.9) 1 0
M 16, 18 1 (0.9) 1 0
M others 3 (2.9) 1 2
Total 107 (100) 67 40
    HC2 HPV detection rate = 67/107 = 62.6%
Samples 513    
%HPV+ 20.9     
  1. M16 = mixed infection with HPV 16 identified by type-specific sequencing primer.
  2. M 16, 18 = mixed infection with HPV 16 and HPV 18 identified by type-specific sequencing primers.
  3. M others = mixed infections by HPV types which cannot be sequenced with type-specific primers for HPV 6, 11, 16 or 18.
  4. Digene High-risk + includes those cases reported as positive for both High-risk and Low-risk HPV types.
  5. The underlined HPV genotypes are included in the "High-risk" Digene HC2 cocktail probe.