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Two cases demonstrate an association between Tropheryma whipplei and pulmonary marginal zone lymphoma

Infectious Agents and Cancer volume 19, Article number: 33 (2024) Cite this article

Abstract

Background

Marginal zone lymphomas of mucosa-associated lymphatic tissues (MZL of MALT) are a group of indolent B-cell neoplasms, which are thought to arise from chronic antigenic stimulation of B-cells either due to underlying chronic infection or autoimmune disease. Little is known about potential causative pathogens in pulmonary MZL (PMZL), although some data suggests a potential role of Achromobacter (A.) xylosoxidans.

Methods

An index case of chronic pulmonary colonisation with Tropheryma (T.) whipplei and subsequent development of PMZL was identified by T. whipplei specific PCR and metagenomic next genome sequencing (mNGS). This case prompted a retrospectively conducted analysis of T. whipplei-specific PCRs in lung tissue from PMZL patients (n = 22), other pulmonary lymphomas, and normal controls. Positive results were confirmed by mNGS. A systematic search for T. whipplei and A. xylosoxidans in our in-house mNGS dataset comprising autopsy lungs, lung biopsies and lung resection specimens (n = 181) was subsequently performed.

Results

A 69-year-old patient presented with weight loss and persistent pulmonary consolidation. Subsequent mNGS analysis detected T. whipplei in the resected lung specimen. An antibiotic regimen eventually eliminated the bacterium. However, the consolidation persisted, and the diagnosis of PMZL was made in a second lung resection specimen. A second case of T. whipplei-associated PMZL was subsequently detected in the retrospectively analysed PMZL cohort. Both cases showed comparatively few mutations and no mutations in genes encoding for NF-κB pathway components, suggesting that T. whipplei infection may substitute for mutations in these PMZL. None of the samples in our in-house dataset tested positive for T. whipplei. In contrast, A. xylosoxidans was frequently found in both autopsy lungs and lung biopsy / resection specimens that were not affected by PMZL (> 50%).

Conclusions

Our data suggests that T. whipplei colonisation of lungs may trigger PMZL as a potential driver. Systematic analyses with larger cohorts should be conducted to further support this hypothesis. The frequent detection of A. xylosoxidans in lung tissue suggests that it is a common component of the pulmonary microbiome and therefore less likely to trigger lymphomas.

Background

In 2018, 2.2 million infection-attributable cancer cases were diagnosed worldwide [1]. To optimise screening programs and antineoplastic therapy in infection-associated subtypes of cancer, it is thus necessary to study the crosstalk of infections and neoplasia. While carcinogenesis caused by viruses such as human papillomavirus (HPV) and Epstein-Barr virus (EBV) is extensively explored, comparatively less is known about the relationship between certain bacterial infections and neoplasia.

Marginal zone lymphomas of mucosa-associated lymphatic tissues (MZL of MALT) are a group of indolent B-cell neoplasms that are thought to arise from chronic antigenic stimulation in mucous membranes, either due to an infectious agent or underlying autoimmune disease [2,3,4]. Certain bacteria are known or suspected to be associated with MZL of MALT lymphomagenesis, e.g. Helicobacter pylori in gastric MZL [5], Chlamydia psittaci in ocular adnexal MZL [6], and Borrelia burgdorferi in cutaneous MZL [7]. The lung parenchyma hosts a dynamic and heterogeneous microbiome, which makes it an interesting source of study for potential lymphomagenic infections [8]. Microbiological studies suggested a potential role of Achromobacter (A.) xylosoxidans in the pathogenesis of pulmonary MZL (PMZL) [9], which was subsequently challenged by metagenomics analyses [10]. Therefore, systematic research is required to identify potential infectious agents which may play a causative role in the pathogenesis of PMZL.

Tropheryma (T.) whipplei, a Gram-positive rod-shaped bacterium of the phylum Actinomycetota, is the causative organism of Whipple’s disease, a systemic disorder affecting the gastrointestinal tract and other organ systems including the cardiovascular system, central nervous system, and joints. The only known reservoir of T. whipplei in humans is the gastrointestinal tract, where the bacteria accumulate in macrophages of the lamina propria [11]. Chronic carrier rate in the population is high (up to 20% in specific populations such as sewage workers, HIV-infected and the homeless [11,12,13]), while the development of the full clinical picture of Whipple’s disease is markedly rare (incidence < 1/1′000′000). This suggests that potential hereditary [14], gender-specific and/or environmental factors [12] additionally play a role in developing symptomatic disease. Typical Whipple’s disease patients are middle-aged Caucasian males who present with diarrhoea, arthralgia, and fever. Chronic disease can manifest in different ways, ranging from endocarditis, uveitis, polyarthritis to encephalitis [15, 16]. Pulmonary infections are rare, though cases of both acute and chronic pneumonia, interstitial lung disease, and pulmonary hypertension in association with T. whipplei have been reported [15, 17]. Isolated reports in the literature have described cases with chronic T. whipplei colonisation associated with B-cell neoplasia [18,19,20].

We report a case of a 69-year-old female with chronic pulmonary Whipple’s disease who subsequently developed PMZL. This case prompted us to systematically analyse a series of PMZL and other pulmonary lymphomas by means of T. whipplei PCR and metagenomic next genome sequencing (mNGS) to explore a potential pathophysiological link between T. whipplei and PMZL. In this analysis, we found a second case of T. whipplei-positive PMZL. This suggests that chronic T. whipplei infections may be associated with PMZL, may even promote lymphomagenesis, and/or that neoplasias such as PMZL may provide a niche for T. whipplei persistence.

Materials and methods

Patient selection

Lung tissue containing PMZL (n = 22), other pulmonary lymphomas (n = 8), and normal lung tissue (n = 3) was retrieved from the archives of the Institutes of Pathology of the University Hospital Basel and of the Cantonal Hospital Baselland, Switzerland (2005–2022) [21]. Clinical data collected for each case include age, sex, date of diagnosis, follow-up, and history of antibiotic intake. At least one representative sample of lung tissue containing PMZL was examined. Due to a potential heterogeneity of microbial load, two or three lung samples from varying anatomical locations were analysed in seven cases (Table 1).

Table 1 Whipple PCRs on a cohort of 22 PZML. In cases with sufficient clinical data, a minimum 7-day intake of antibiotics within a period of 4 weeks prior to tissue sampling was documented
Full size table

Immunohistochemistry

Immunohistochemical double stains for PAX5 (rabbit anti-human PAX5, clone SP34, 312R, dilution 1:25, incubation 15 min; Cellmarque, Sigma-Aldrich, Rocklin, CA, USA; Bond Polymer Refine Detection DS9800), and the pan-cytokeratin AE1/AE3 (mouse anti-human cytokeratin, clone AE1/AE3, M3515, dilution 1:100, incubation 15 min; Agilent, Santa Clara, CA, USA; Bond Polymer Refine Red Detection DS9390) were performed on a Leica Bond III autostainer using EDTA buffer (pH9) for antigen retrieval (H2(30)95, AR9940) and the REFINE polymer detection system (Leica Biosystems, Newcastle, UK).

Immunohistochemistry for CD1a (mouse anti-human CD1a, clone 010, M3571, dilution 1:100, incubation 20 min; Agilent, Santa Clara, CA, USA; Bond Polymer Refine Red Detection DS9390) was also performed on a Leica Bond III autostainer using EDTA buffer (pH9) for antigen retrieval (H2(20)100, AR9940) and the REFINE polymer detection system (Leica Biosystems, Newcastle, UK).

Nucleic acid extraction

For PCR and mNGS analyses, ten formalin-fixed, paraffin-embedded (FFPE) tissue sections were incubated with proteinase K overnight. DNA was extracted on a Qiagen EZ2 Connect device with the Qiagen EZ1&2 DNA Tissue kit (Qiagen, Hilden, Germany).

For high-throughput sequencing (HTS), DNA extraction from 10 µm thick untreated FFPE tissue was performed using the Invitrogen™ RecoverAll™ Multi-Sample RNA/DNA Workflow (#A26069, Thermo Fisher Scientific, Waltham, MA, USA).

Polymerase chain reactions

PCR was performed with the Ampli-Taq Gold polymerase (Thermo Fisher Scientific, Waltham, MA, USA) with two sets of primers specifically targeting the 16 s and 23 s rRNA of T. whipplei (16 s rRNA primers: 5’-AGAGATACGCCCCCCGCAA-3’ and 5’-TCCTGTGAGTCCCCGCCATTACGC-3’ with an annealing temperature of 55 °C; 23 s rRNA primers: 5’-GGGTAGTGGGATCCTCTATGTGG-3’ and 5’-CGGGACTATCACCCTCTTCG-3’ with an annealing temperature of 55 °C). PCR products (with expected sizes of 141 and 101 base pairs, respectively) were resolved using a Qiagen Qiaxcel Connect capillary electrophoresis device.

The presence of Mycobacterium tuberculosis and Mycobacterium species (including M. marinum, leprae, kansasii, lufu and haemophilum) was assessed by a nested PCR targeting the IS6110 and a quantitative PCR targeting additional sequences (see Supplementary Table 1 for primer and probe sequences).

The presence of Francisella tularensis was determined via a semi-nested PCR using the following primers: primer 1–5’-TGCTGCTGCTCAGACAGCTACTA-3’, primer 2–5’-ACCTTCTGGAGCCTGCCATTGT-3’, primer 3–5’-GCAAGCTGCTGCTGTATCTAAGCCA-3’. An annealing temperature of 64 °C and the GeneAmp Fast PCR Master Mix (Thermo Fisher Scientific, Waltham, MA, USA) were used.

All PCR performed included a synthetic positive, negative, and extraction control (a kidney tissue FFPE block negative for T. whipplei and with good DNA quality). DNA quality was assessed in each sample via a human β-Globin PCR.

Metagenomic next-generation sequencing (mNGS)

Shotgun metagenomic sequencing was used to sequence the microbial content of the samples without prior selection nor enrichment. NGS libraries using 250 ng of DNA from each sample were generated using the Ion Plus Fragment Library kit (Thermo Fisher Scientific, Waltham, MA, USA). Libraries were quantified using the Ion Library TaqMan Quantification kit (Thermo Fisher Scientific, Waltham, MA, USA). Sequencing was performed on an Ion Torrent GeneStudio S5XL (Thermo Fisher Scientific, Waltham, MA, USA).

Pathogen detection by mNGS

The shotgun sequencing data was analysed with the CLC Genomics Workbench software and its Microbial Genomics module (Qiagen, Hilden, Germany) to perform taxonomic profiling and pathogen detectionas previously described [22].

High throughput sequencing and data analysis

Mutational analysis by HTS was performed using an IonAmpliSeq™-customised, -validated and ISO15189-accredited lymphoma panel comprising 4716 amplicons (size range: 125–175) of 172 genes (Thermo Fisher Scientific, Waltham, MA, USA) [23]. Library preparation, chip loading, variant annotation and variant classification were conducted as previously described [24].

Data availability statement

The datasets featured in this study are accessible via the European Nucleotide Archive (ENA, https://www.ebi.ac.uk/) under the accession no. PRJEB73816.

Results

Index patient

A 69-year-old female (subsequently referred to as “index patient”) first presented to our outpatient clinic in August 2019 with extensive weight loss (20 kg over the course of 5 months). A CT scan revealed a consolidation in the right lower pulmonary lobe. No dyspnoea or cough was reported. The patient had stopped smoking 50 years ago and has a history of 10 pack years. Bronchoalveolar lavage (BAL) and transbronchial biopsy yielded no evidence of malignancy. Cytologic examination of bronchial secretion and BAL showed mixed inflammation with a predominance of neutrophilic granulocytes, while in bronchial fluid, granulomatous inflammation was described. However, PCR analyses for Mycobacterium spp. and Francisella tularensis yielded negative results.

The patient first refused antibiotic therapy. In September 2020, a treatment regimen with corticosteroids was initiated (prednisolone 75 mg for 4 weeks; tapering of dosage until December 2020). Despite treatment, the consolidation and symptoms persisted, and the right lower lobe was partially resected (March 2021). Histology revealed an acccumulation of alveolar macrophages, intra-alveolar granulomas with giant cells, and prominent peribronchiolar metaplasia. No intracytoplasmic periodic acid Schiff (PAS) positive granules were detected in alveolar macrophages. Langerhans cell histiocytosis was ruled out by immunohistochemistry (negativity for CD1a). PCR for Mycobacterium spp. and 44 other respiratory pathogens in the resection specimen was similarly negative. A mNGS analysis was performed, which detected T. whipplei. Subsequent retrospective PCR analyses also detected T. whipplei in all previously analysed cytology and biopsy specimens (see Fig. 1 for a full timeline of events). An oesophago-gastro-duodenoscopy was performed, which was unremarkable histologically and yielded negative PCR results for T. whipplei. This suggested the lung as the only T. whipplei reservoir in this patient. The patient decided against a cerebrospinal fluid analysis, but agreed to antibiotic treatment in November 2022 (initially doxycycline and hydroxychloroquine, later switched to cotrimoxazole and ceftriaxone due to gastrointestinal side effects, Fig. 1).

Fig. 1
figure 1

Five-year timeline of clinical events for the index patient with PMZL and detection of T. whipplei in various lung tissues using molecular methods (T. whipplei specific PCR, mNGS)

Full size image

The pulmonary consolidation persisted and progressed further, and in March 2023 total lobectomy was performed. Gross analysis of the resection specimen revealed an inhomogeneous consolidation of the lung parenchyma (Fig. 2a). Histological analysis diagnosed a small cell B-cell lymphoma, consistent with PMZL. The surrounding pulmonary parenchyma was remarkable for numerous epithelioid granulomas with giant cell formation as well as perifocal organising pneumonia (Fig. 2b–f). The lymphoma was limited to the lung parenchyma without locoregional spread to perihilar lymph nodes. Of note, a PCR for T. whipplei was negative on this resection specimen (4 months after the start of antibiotic therapy).

Fig. 2
figure 2

Gross appearance (a) and histological findings (b-f) of the lung of the index patient. a Gross analysis of the lung resection specimen shows an inhomogeneous consolidation of the parenchyma (beige). b, c Microscopic findings denote a dense lymphocytic infiltrate consisting of monomorphic small lymphocytes (H&E). d Double staining for PAX5 (brown) and a pan-cytokeratin (AE1/AE3, red) demonstrate lymphoepithelial lesions. e Numerous intra- and perilesional epithelioid cell granulomas with multinucleated giant cells are similarly observed in adjacent parenchyma (f) (H&E). b Scale bar: 500 µm. c–f Scale bars: 50 µm

Full size image

Eleven days after the operation, the patient presented with a persistent air fistula, which was surgically revised. The patient was dismissed 20 days after admission. She continued antibiotic treatment for 12 months and underwent serological follow-ups every three months. In the last follow-up in March 2024, no peculiarities were reported, and the most recently performed CT and PET scans did not display evidence of a relapse.

Analysis of a cohort of PMZL and other lymphomas

As we followed the case of this patient longitudinally for several years, we hypothesised that T. whipplei may have been a chronic infectious stimulus that promoted the development of PMZL in this patient. Therefore, lung biopsies from a larger cohort of PMZL patients (n = 22) were screened for T. whipplei by PCR (Table 1) [21]. In this cohort, one other case with T. whipplei positivity in two lung samples, obtained at two different time points, was detected (subsequently referred to as “patient 2”). This result was confirmed by a mNGS analysis.

Patient 2 was a 36-year-old male patient with a migration background. He had been suffering from tuberculosis abroad and sought medical advice in Switzerland for persistent cough (6 months). Imaging showed parenchymal scarring and bilateral pulmonary consolidation suggestive of sarcoidosis, Langerhans cell histiocytosis, or an interstitial lung disease. He was initially treated with broad-spectrum antibiotics (co-amoxicillin and clarithromycin) for 9 days in May 2008. As the consolidations persisted, lung biopsies were taken in July 2008 which showed marked peribronchial and paraseptal lymphoplasmacytic aggregates suspicious for PMZL. Subsequently, the patient developed a pneumothorax and underwent thoracoscopic surgery. Perioperative antibiotic prophylaxis was administered (co-amoxicillin). During the operation, the gross appearance of the lungs (extensive bullae and pleural thickening) warranted two wedge resections. Histological analysis revealed dense infiltrates of atypical small B lymphocytes and evidence of typical lymphoepithelial lesions, consistent with PMZL. Adjacent pulmonary tissue demonstrated granulomatous inflammation with multinucleated giant cells (Supplementary Fig. 1). After the diagnosis, the patient was treated with steroids. This patient had no documented steroid intake prior to thoracoscopic tissue resection. At the point PMZL was diagnosed, M. tuberculosis was no longer detected in his lungs (3 samples from distinct anatomical locations were tested by PCR for M. tuberculosis and atypical mycobacteria).

Gastric biopsies were taken as part of PMZL staging, which similarly showed infiltrates of MZL. Although Helicobacter bacteria were not present, eradication therapy was initiated. T. whipplei specific PCRs carried out retrospectively on these gastric biopsies were negative.

For both cases of T. whipplei associated PMZL, HTS was performed as previously described (Supplementary Table 2) [21, 25]. The index patient showed a somatic frame-shift insertion mutation in FOXC1 which was classified as variant of unknown significance (VUS). Patient 2 was found to have a somatic frame-shift insertion mutation in the tumour suppressor gene EP300, classified as likely pathogenic. This patient had an additional missense mutation in SPEN classified as VUS (Supplementary Table 2). Both mutations of the patient 2 had been found in a previously analysed cohort of PMZL cases from our group [25]. In neither case mutations affecting the nuclear factor Kappa B (NF-κB) or the Notch signalling pathway, both known to be frequently altered in MZL, was detected. The number of mutations detected in both cases of T. whipplei associated PMZL was within the range of our previous cohort [21, 25].

All other cases in the PMZL cohort tested negative for T. whipplei (Table 1). However, a subset of patients had received broad-spectrum antibiotic treatment regimens for suspected infections within four weeks before undergoing surgery (4/22 cases), and this may have caused false negative PCR results. In addition, all cases with other pulmonary lymphomas (Supplementary Table 3) and normal lung tissue (Supplementary Table 4) were negative for T. whipplei by PCR. Similarly, a retrospective analysis of our own metagenomics dataset [22, 26] from routinely analysed lung samples (n = 181, of which 115 autopsy lungs and 66 lung biopsies) for T. whipplei yielded negative results. We thus deduce that chronic T. whipplei infection or chronic persistence of T. whipplei in human lungs is very rare. In contrast, A. xylosoxidans bacteria was commonly found in both autopsy and biopsy specimens in > 50% of cases (Fig. 3a, b). This result suggests that A. xylosoxidans is part of the normal lung microbiome and unlikely to play a role in the pathogenesis of PMZL.

Fig. 3
figure 3

Retrospective analysis of our in-house mNGS dataset a consisting of routinely analysed autopsy lungs, lung biopsies and resection specimens (n = 181, of which 115 autopsy lungs and 66 lung biopsies/resections). T. whipplei was not detected in any of the previously analysed lung tissues. b In contrast, A. xylosoxidans was detected in both autopsy and biopsy / resection lung specimens in > 50% of cases, suggesting that it is a common component of the pulmonary microenvironment

Full size image

Discussion

In this study, we present novel evidence for an association between chronic pulmonary T. whipplei infection and PMZL. Pulmonary T. whipplei infection may promote neoplastic transformation of B cells, or neoplastic B cell proliferations may provide a niche for T. whipplei persistence in the lung. In fact, individual cases have been described in the literature that suggest a pathophysiological connection between T. whipplei and B-cell lymphomas, in line with our hypothesis [17,18,19,20, 27].

The multifaceted nature of chronic Whipple’s disease is likely due to a combination of factors, both host- and pathogen-dependent, which ultimately result in a failure to clear the bacterium, leading to chronic intracellular oxidative stress and failed antigen presentation. Host-dependent factors include an increased likelihood to develop chronic Whipple’s disease or persistence in patients with HLA-B27 positivity [28], suggesting an impairment of cell-mediated immunity [17]. Macrophage dysfunction has been postulated to be a hallmark of chronic Whipple’s disease, for example due to a decrease of interleukin-12 (IL-12), disturbed phagocytosis and antigen presentation. This, in turn, decreases interferon gamma (IFNγ), dysregulates NK-, T- and B-cell response [14, 29, 30], and ultimately contributes to lymphomagenesis [10]. Interestingly, clinical evidence suggests that patients with a history of immunosuppression or receipt of immunosuppressive agents, especially TNFα inhibitors, were more likely to develop systemic Whipple’s disease with rapid deterioration directly or soon after medication onset [31,32,33]. Although none of the patients tested positive for T. whipplei in our cohort has a known prior history of immunosuppression, it should be considered as one of the potential risk factors for systemic disease.

Another important factor in the pathogenesis of chronic Whipple’s disease is NF-κB-mediated signalling, downstream of bacterial sensors such as Toll-like receptor 4 (TLR4) [34]. Previous studies suggest that bacterial infections may have activating effects on the B-cell receptor (BCR) and the NF-κB pathway in B cells, further supporting the pathophysiological role of chronic antigen stimulation in lymphomagenesis [35]. It has been shown that NF-κB-mediated oxidative stress can induce de novo genomic rearrangements, as was previously shown for the TMPRSS2-ERG gene fusion in prostate cancer [36]. The role of NF-κB in PMZL lymphomagenesis is also supported by HTS analyses [21], which identified mutations and translocations of TNFAIP3, BCL10, CARD11, MYD88, TNFRSF14 and MALT1 in almost 60% of PMZL. All of these alterations have been shown to influence the activity of the NF-kB pathway [37, 38]. Interestingly, both cases of T. whipplei-associated PMZL showed comparatively few mutations and, in particular, no mutations in genes related to the NF-κB or the Notch signalling pathway and no mutations in chromatin modifier encoding genes characteristic for PMZL [21, 25]. A similar pattern is observed in diffuse large B-cell lymphoma (DLBCL), where the mutational profiles and load differ significantly between EBV-positive and negative cases [39, 40]. These findings suggest that T. whipplei infection may compensate for the missing mutations in these PMZL, thus promoting lymphomagenesis.

Our data on the association of T. whipplei and PMZL suggests a potential causative link between T. whipplei infection and PMZL requiring further confirmative studies. MZL in other localisations are similarly associated with various chronic infections [5,6,7], and patients with PMZL have been successfully treated with prolonged clarithromycin therapy, further corroborating a potential infectious cause of this B-cell lymphoma [41]. However, several uncertainties remain. Patient 2 had a history of M. tuberculosis, which might have contributed to the development of PMZL through chronic inflammatory stimulation. Only a minority of pathogens are known to chronically persist in the lung, which is a fundamental prerequisite for chronic inflammatory stimulation. Indeed, M. tuberculosis DNA has been found by in-situ PCR within lungs of individuals who have died from causes other than tuberculosis [42, 43]. A previously reported case suggested a temporal link between the occurrence of PMZL and pulmonary mycobacterial infection and regression of PMZL after antibiotic treatment for concomitant pulmonary tuberculosis [44]. Based on this data, we cannot exclude a pathophysiological connection between chronic mycobacterial infection and the occurrence of PMZL in patient 2. However, a PCR on the lung tissue taken at the time of PMZL diagnosis was negative for mycobacteria (M. tuberculosis and atypical mycobacteria).

A history of perioperative broad-spectrum antibiotic treatment in 4 of 22 cases might have influenced the negative PCR results of the cohort, although the rate of tissue penetration of differing antibiotic regimens should not be overestimated. In general, antibiotic intake can cause false negative results in PCR analyses, as demonstrated in the second lung resection specimen of the index patient, which turned out negative T. whipplei after four months of antibiotic treatment (Fig. 1). The elimination of causative bacterial species by means of antibiotic therapy could eventually become a component of PMZL treatment, similar to Helicobacter eradication in gastric MZL [45,46,47,48]. As other bacterial infections could be potential PMZL drivers, it will be important to study larger PMZL cohorts with a non-targeted approach such as mNGS, rather than by means of species-specific PCR only.

Non-targeted analyses conducted so far have shown no evidence of any causative link between any pathogen and PMZL and, in particular, have refuted the suggested role of A. xylosoxidans [10]. Our data supports A. xylosoxidans as a component of the normal lung microbiome and do not suggest it as a potential contaminant. Firstly, its pattern of detection is neither systematic nor isolated. In contrast, our dataset is systematically contaminated by Sphingomonas spp. (plant-related bacteria) and occasionally displays abundant contamination by Methylobacterium spp., which are known to contaminate DNA extraction reagents [49]. In contrast, A. xylosoxidans is evenly represented in our cohort. Secondly, we have recently implemented improvements to our bioinformatics pipeline based on the latest developments and recommendations in the field of metagenomics [50, 51]. We now perform a second human read depletion using the latest and complete version of the human genome (CHM13). This additional filtering has enabled us to identify and remove contaminants upon reanalysis of our data. A. xylosoxidans was not identified as a bioinformatics artefact during this process, further suggesting its actual presence in our samples.

Conclusions

Our data highlights a potential role of T. whipplei infection in pulmonary granulomatous lung disease with an unclear aetiology and establishes a potential association between this bacterium and PMZL. In addition, our study emphasises the utility of metagenomics in daily pathology practice, which could significantly impact on the treatment regimen of PMZL. This introduces the possibility of first-line antibiotic therapy in cases of PMZL where T. whipplei is detected. Future studies featuring larger cohorts of patients with unclear granulomatous lung disease and/or PMZL screened for T. whipplei are required to further strengthen the hypothesis that T. whipplei may be a potential driver of PMZL.

Availability of data and materials

The HTS data acquired for this study is accessible via the European Nucleotide Archive (ENA, https://www.ebi.ac.uk/) under the accession no. PRJEB73816.

Abbreviations

BAL:

Bronchoalveolar lavage

BCR:

B cell receptor

CT:

Computed tomography

DLBCL:

Diffuse large B-cell lymphoma

EBV:

Epstein-Barr virus

EDTA:

Ethylenediaminetetraacetic acid

FFPE:

Formalin-fixed paraffin-embedded

HIV:

Human immunodeficiency virus

HLA:

Human leukocyte antigen

HPV:

Human papilloma virus

HTS:

High-throughput sequencing

IFNγ:

Interferon gamma

IL:

Interleukin

KMT2D:

Histone-lysine N-methyltransferase 2D

MALT:

Mucosa-associated lymphatic tissue

MZL:

Marginal zone lymphoma

NF-kB:

Nuclear factor Kappa B

mNGS:

Metagenomic next-generation sequencing

NK:

Natural killer

PAS:

Periodic acid Schiff

PCR:

Polymerase chain reaction

PET:

Positron emission tomography

PMZL:

Pulmonary marginal zone lymphoma

rRNA:

Ribosomal ribonucleic acid

spp.:

Species

TGFβ:

Transforming growth factor Beta

TLR4:

Toll-like receptor 4

TMPRSS2-ERG:

Transmembrane serine protease 2/ETS transcription factor

TNFAIP3:

Tumour necrosis factor, alpha induced protein 3

VUS:

Variant of unknown significance

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Acknowledgements

The authors would like to thank all patients participating in this study and their relatives.

Funding

Open access funding provided by University of Basel. Open access funding provided by University of Basel.

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Author notes

  1. J. D. Haslbauer, C. Wiegand, and B. Hamelin jointly contributed to this work.

Authors and Affiliations

  1. Institute of Medical Genetics and Pathology, University Hospital Basel, Basel, Switzerland

    J. D. Haslbauer, V. S. Ivanova, T. Menter, S. Savic Prince & A. Tzankov

  2. Institute of Pathology, Cantonal Hospital Baselland, Mühlemattstrasse 11, CH-4410, Liestal, Switzerland

    C. Wiegand, B. Hamelin & K. D. Mertz

  3. University of Basel, Basel, Switzerland

    A. Tzankov & K. D. Mertz

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  1. J. D. Haslbauer
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Contributions

JDH, CW and BH contributed equally to this work. Data acquisition and histological analysis by JDH, CW, VSI, AT, SS, KDM. PCR and mNGS assays by BH. Clinical information by JDH and CW. Manuscript by JDH, CW, BH and KDM. Critical appraisal of the manuscript by VSI, TM, SS, AT, KDM.

Corresponding author

Correspondence to K. D. Mertz.

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Ethics approval and consent to participate

This study was conducted according to the principles of the Declaration of Helsinki. The study protocol was reviewed and approved by the Ethics Committee of Northwestern and Central Switzerland (Project-ID 2023–02250). Patient consent was obtained for sample collection and research use.

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The authors declare no competing interests.

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Haslbauer, J.D., Wiegand, C., Hamelin, B. et al. Two cases demonstrate an association between Tropheryma whipplei and pulmonary marginal zone lymphoma. Infect Agents Cancer 19, 33 (2024). https://doi.org/10.1186/s13027-024-00597-0

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Keywords

Infectious Agents and Cancer

ISSN: 1750-9378

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