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Fig. 2 | Infectious Agents and Cancer

Fig. 2

From: EPRS1 correlates with malignant progression in hepatocellular carcinoma

Fig. 2

EPRS1 knockdown depressed malignant phenotypes. A, Detection of relative EPRS1 mRNA expression in five HCC cell lines. The cell line with the lowest EPRS1 mRNA level was set to 1. B, siRNAs were employed to knock down EPRS1 expression. EPRS1 downregulation confirmed at mRNA level in Hep3B cells. C, Validation of EPRS1 downregulation at the protein level by Western blot. Predicted EPRS1 molecular mass: 170.6 kDa. D, Proliferation ability of Hep3B cells after siRNA knockdown of EPRS1 expression was assessed at the indicated time points (n ≥ 6 for each time point) by CCK8 assay. E, Growth curves of Hep3B and Huh7 cells under halofuginone treatment measured by CCK-8 assay. F, Transwell migration assay on cells treated with siRNA targeting EPRS1. F-i, Representative images of membranes with violet spots indicating migrated cells stained with crystal violet. F-ii, Statistical analysis of migratory Huh7 (left) and Hep3B (right) cell numbers. G, Migration assay on cells treated with 100 nmol/L halofuginone (Halo) for 48 h. G-i, Representative images of migrated cell spots. G-ii, Statistical analysis of migratory Huh7 (left), HepG2 (middle), and Hep3B (right) cell numbers. H, Hepatosphere formation assay on cells treated with siRNA targeting EPRS1. H-i, Representative images of hepatospheres. H-ii, Quantification of hepatospheres formed by Huh7 (left) and Hep3B (right) cells after EPRS1 silencing

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