Primers | 5′-3′ sequences | PCR product | PCR conditions | References |
---|---|---|---|---|
HPV-31 (F) HPV-31 (R) | CCTACAACGCCACAAGTGTC CAATACAGCACAAGCACATACAC | 1667 bp | Primary denaturation: 95 °C, 120 s Denaturation: 95 °C, 20 s Annealing: 58 °C, 25 s × 43 Extension: 72 °C, 85 s Final extension: 72 °C, 300 s | This study |
HPV-33 (F) HPV-33 (R) | ACCATTGTTGTAGACGGTGC GACAAGTACATAGAACATGCACAC | 1760 bp | Primary denaturation: 95 °C, 120 s Denaturation: 95 °C, 20 s Annealing: 56 °C, 25 s × 43 Extension: 72 °C, 95 s Final extension: 72 °C, 300 s | This study |
HPV-58 (F) HPV-58 (R) | CGTACCAGTAATGTGTCCATACC GCACCTTACTCATAGATACACCC | 1950 bp | Primary denaturation: 95 °C, 120 s Denaturation: 95 °C, 20 s Annealing: 60 °C, 25 s × 43 Extension: 72 °C, 105 s Final extension: 72 °C, 300 s | This study |