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Table 1 The primers and programs of the thermocycler with temperatures cycle

From: Genetic diversity and bioinformatic analysis in the L1 gene of HPV genotypes 31, 33, and 58 circulating in women with normal cervical cytology

Primers

5′-3′ sequences

PCR product

PCR conditions

References

HPV-31 (F)

HPV-31 (R)

CCTACAACGCCACAAGTGTC

CAATACAGCACAAGCACATACAC

1667 bp

Primary denaturation: 95 °C, 120 s

Denaturation: 95 °C, 20 s

Annealing: 58 °C, 25 s × 43

Extension: 72 °C, 85 s

Final extension: 72 °C, 300 s

This study

HPV-33 (F)

HPV-33 (R)

ACCATTGTTGTAGACGGTGC

GACAAGTACATAGAACATGCACAC

1760 bp

Primary denaturation: 95 °C, 120 s

Denaturation: 95 °C, 20 s

Annealing: 56 °C, 25 s × 43

Extension: 72 °C, 95 s

Final extension: 72 °C, 300 s

This study

HPV-58 (F)

HPV-58 (R)

CGTACCAGTAATGTGTCCATACC

GCACCTTACTCATAGATACACCC

1950 bp

Primary denaturation: 95 °C, 120 s

Denaturation: 95 °C, 20 s

Annealing: 60 °C, 25 s × 43

Extension: 72 °C, 105 s

Final extension: 72 °C, 300 s

This study