Fig. 6

Apoptosis induced by ATF3 overexpression on Ca Ski cells. The histogram represents the percentage of total apoptotic cells in different groups. FlowJo software version 10.0 was used to draw the flow cytometry plots. Ca Ski cells were transfected with 7 µg of pCMV6-ATF3 prior to the measurement of apoptosis level using PE Annexin V apoptosis detection kit after 48 h (A). A statistically significant increase in the number of apoptotic Ca Ski cells was observed when compared to the ‎untreated and ‎mock groups (p < 0.0001). However, the difference in the number of apoptotic cells between the ‎untreated and ‎mock group was not significant ‎(p > 0.4) (B). Q1, Q2, Q3, and Q4 indicate necrotic, late apoptotic, early apoptotic, and viable cells, respectively. The percentage of total apoptotic cells was calculated from Q2 + Q3.