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Table 2 Detection of EBV genome by applying conventional (EBER-ISH) and unconventional methods (ddPCR, RNAscope) in two different histological types of GC (ADK, GCLS)

From: EBV persistence in gastric cancer cases conventionally classified as EBER-ISH negative

Case Number

 

Assay

Histological Type

EBER-ISH

ddPCR

RNAScope

EBERs

BamHI (copies/ul)

EBNA1 (copies/ul)

EBNA1 mRNAs (score)

 

#1

2

0

ADK

#2

0

0

#3

 + 

988

1287

2500

#4

0

0

#5

0

0

#6

0

0

#7

0

0

#8

0

0

#9

0

0

#10

0

0

#11

2.2

0

#12

9.8

1.5

8

#13

0

0

#14

0

0

#15

1.4

0

6

#16

0.3

0

#17

0.5

0

#18

0

0

#19

0

0

#20

0

0

#21

1.5

0.9

8

#22

0

0

#23

0

0

#24

5.7

0

7

#25

0

0

#26

0

0

#27

0

0

#28

 + 

1202

1314

2600

HCL

 

0

0

 

HCL

 

0

0

 

HCL

 

0

0

 

#29

0.52

0.17

GCLS

#30

41.85

4

10

#31

19.1

2.03

8

#32

3.23

0.53

12

#33

0.94

0.48

#34

0.46

0.18

#35

0

0

#36

 + 

6460

2847

3000

#37

 + 

1410

5100

2900

#38

 + 

2710

1351

np

#39

 + 

466

152

np

#40

 + 

2089

587

np

HCL

 

0

0

 

HCL

 

0

0

 

HCL

 

0

0