Fig. 2

Electrophoresis of PCR–RFLP products of LTAg region of human polyomaviruses genome extracted from bladder tissue samples on 2% agarose gel to distinguish between BKPyV and JCPyV. M, 50-bp DNA ladder; Ca and Cb, Negative controls; 1a, 2a, 3a, 4a, 5a, 6a, 7a and 8a, PCR products after BamHI endonuclease digestion; 1b, 2b, 3b, 4b, 5b, 6b, 7b and 8b, PCR products before digestion; 1a, 3a, 4a, 5a, 7a, Undigested PCR amplicons of BKPyV by BamHI restriction endonuclease (176 bp length fragments); 2a, 3a, 4a, 6a, 8a, Digested PCR amplicons of JCPyV by BamHI restriction endonuclease (122 bp and 54 bp length fragments); 3a and 4a, BKPyV and JCPyV coinfection; Ca and Cb, Negative controls; M, 100-bp DNA ladder