Fig. 5

GP73 facilitates CSC generation in HepG2 and Huh7 cells. A HepG2-GFP and HepG2-GP73 cells were cultured in ultralow attachment 6-well plates at 2000 cells/well density. The sphere formation ability of the HepG2-GFP and HepG2-GP73 cells was investigated. B Huh7-GFP and Huh7-GP73 cells were sorted into ultralow attachment 96-well plates at a density of 1 cell/well by flow cytometry. The spheroid phenotype changes and numbers of the HepG2-GFP and HepG2-GP73 cells were examined by microscopy. C A wound-healing assay to test the migration of L02-GP73 and L02-GFP cells is shown (scale bar = 100 μm). The graphs show the means ± SDs, n = 3. *P < 0.05, **P < 0.01, compared with the control group