Fig. 2From: Hepatitis B virus promotes hepatocellular carcinoma development by activating GP73 to repress the innate immune responseHBV induces activation of GP73. A pHBV1.3 at different doses was transfected into HepG2 and Huh7 cells. After 48 h, HBsAg and HBeAg in cell culture supernatants were detected by ELISAs. B pHBV1.3 at different doses was transfected into HepG2 and Huh7 cells. After 48 h, the expression of GP73 was detected by qRT-PCR. C, D The pHBV1.3 overexpression plasmid (0.4 μg/ml) was transfected into primary human hepatocytes (PHHs). The expression of HBsAg and GP73 was detected by Western blots (C). The expression of GP73 was analyzed by qRT-PCR. D The graphs show the means ± SDs, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, compared with the control groupBack to article page