Fig. 3

MiRNA expression analysis performed in a K49, K49 sil., EV_K49, EV_K49 sil. and b K16, K16 sil., EV_K16, EV_K16 sil.. Total RNA from each sample was purified as described in Materials and Methods. RNU6 was used as a calibrator and K49 sil., EV_K49 sil., K16 sil. and EV_K16 sil. were used as controls. Results were expressed as fold of induction or decrease using the 2^−ΔΔCT method [28]. The reported values represent the mean of three independent experiments ± the standard deviation. P-values (P) are indicated with asterisks. **P ≤ 0.01, *P ≤ 0.05