Fig. 3

Effects of Wnt/β-catenin signaling pathway on proliferation and activation of HSC-T6 cells. A The protein expression of Wnt3a, β-catenin and p-GSK3β from rat liver tissues was detected by western blotting. B The cell viability of HSC-T6 cells was measured by CCK-8 analysis. C The cell cycle was detected by flow cytometry analysis of HSC-T6 cellsp. D The protein expression of α-SMA, Collagen I, cyclin-D1, c-Myc, MMP7, Wnt3a, β-catenin and p-GSK3β in HSC-T6 cells was detected by western blotting. Data were presented as the mean ± SD obtained from three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001