Fig. 2

BMSCs suppresses TGF-β1-induced HSC-T6 activation. A The mRNA expression of α-SMA and Collagen I in HSC-T6 cells were detected by RT-qPCR after treatment with TGF-β1. B The cell viability of HSC-T6 cells was measured by CCK-8 analysis. C The cell cycle was detected by flow cytometry analysis of HSC-T6 cells in each group. D The protein expression of α-SMA, Col1α1, cyclin-D1, c-myc, and MMP7 from HSC-T6 cells was detected by western blotting. E The protein expression of cyclin-D1and c-myc from rat liver tissues was detected by western blotting. Data were presented as the mean ± SD obtained from three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001